Lee Taek, Kim Sang-Uk, Lee Jin-Ho, Min Junhong, Choi Jeong-Woo
Department of Chemical and Biomolecular Engineering, Sogang University, #1 Shinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea.
J Nanosci Nanotechnol. 2009 Dec;9(12):7136-40. doi: 10.1166/jnn.2009.1663.
The biomolecular/organic hetero-structure films (cytochrome c/11-mercapto-undecanoic acid) on gold substrates were controlled and fabricated with molecular level for developing valuable molecular electronic devices. Cytochrome c is a metalloprotein having redox property, which can be directly applicable to biomemory device as a active element. For efficient immobilization of the protein on the gold substrate, 11-mercapto-undecanoic acid (11-MUA) was used as a linker material between protein and inorganic substrate. The proposed nano scaled biomolecular/organic hetero-structure layer (cytochrome c/11-MUA) on gold surface was investigated by using surface plasmon resonance technique. The molecular morphology of the fabricated protein layer was confirmed by scanning tunneling microscopy. Electrochemical properties of fabricated biomolecular/organic hetero layer were verified using cyclic voltammetry. Their redox properties was sustained over 1000 cycles of cyclic voltametry. It proved that the fabricated film was a suitable platform for the bioelectronic device application.
为了开发有价值的分子电子器件，对金基底上的生物分子/有机异质结构薄膜（细胞色素c/11-巯基十一烷酸）进行了分子水平的控制和制备。细胞色素c是一种具有氧化还原特性的金属蛋白，可作为活性元件直接应用于生物存储器件。为了将蛋白质有效地固定在金基底上，11-巯基十一烷酸（11-MUA）被用作蛋白质与无机基底之间的连接材料。利用表面等离子体共振技术对金表面提出的纳米级生物分子/有机异质结构层（细胞色素c/11-MUA）进行了研究。通过扫描隧道显微镜确认了制备的蛋白质层的分子形态。利用循环伏安法验证了制备的生物分子/有机异质层的电化学性质。其氧化还原性质在1000次循环伏安法中得以保持。这证明所制备的薄膜是生物电子器件应用的合适平台。
