Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 121-742, Republic of Korea.
J Biomed Nanotechnol. 2013 May;9(5):849-55. doi: 10.1166/jbn.2013.1496.
We fabricated and analyzed a nanoscale biofilm of human lactoferrin making use of 11-mercapto-undecanoic acid (11-MUA) as chemical linker. The fabrication of the bimolecular/organic hetero monolayer (lactoferrin/11-MUA) on gold substrate was confirmed with Raman spectroscopy. Cyclic voltammetry (CV) was carried out to observe the electrochemical properties of the nanoscaled biofilm under various pH conditions and at different time intervals. The well-defined redox properties were observed, even in certain harsh pH conditions and after a long time, proving the stabilities of this biofilm. Atomic force microscopy (AFM) was further employed to confirm the retention time by investigating the morphology variety of the biofilm over time. All these results proved that, the proposed nanoscaled thin film composed of lactoferrin and 11-MUA is a powerful alternative for making bioelectronics devices.
我们利用 11-巯基十一酸（11-MUA）作为化学连接物，制备和分析了人乳铁蛋白的纳米级生物膜。拉曼光谱证实了双分子/有机杂化单层（乳铁蛋白/11-MUA）在金基底上的形成。循环伏安法（CV）用于观察在不同 pH 值条件下和不同时间间隔下纳米生物膜的电化学性质。即使在某些苛刻的 pH 值条件下和很长时间后，仍观察到明确的氧化还原性质，证明了该生物膜的稳定性。原子力显微镜（AFM）进一步用于通过研究生物膜随时间的形态变化来确认保留时间。所有这些结果都证明，由乳铁蛋白和 11-MUA 组成的所提出的纳米薄膜是制造生物电子器件的有力替代品。
