Lee Taek, Min Junhong, Lee Jin-Ho, Choi Jeong-Woo
Department of Chemical and Biomolecular Engineering, Sogang University, #1 Shinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea.
J Nanosci Nanotechnol. 2011 Jan;11(1):523-7. doi: 10.1166/jnn.2011.3272.
A multi-bit biomemory device was devised by introducing 4 different metalloproteins (azurin, cytochrome c, ferredoxin, myoglobin) to an electronic device using 11-MUA (11-mercapto-undecanoic acid) as the chemical linker. The immobilization of the 4 different self-assembled protein layers on a Au substrate via 11-MUA were confirmed by Raman spectroscopy and atomic force microscopy (AFM). The redox properties of these 4 different protein layers immobilized onto Au surface were assessed by cyclic voltammetry (CV). In addition, their memory functions were verified by chronoamperometry (CA). Based on these results, we demonstrated that a multi-bit biomemory concept could be realized using various metalloproteins as active materials.
通过使用11-巯基十一烷酸（11-MUA）作为化学连接剂，将4种不同的金属蛋白（天青蛋白、细胞色素c、铁氧化还原蛋白、肌红蛋白）引入电子设备，设计出了一种多位生物记忆器件。通过拉曼光谱和原子力显微镜（AFM）证实了这4种不同的自组装蛋白层通过11-MUA固定在金基底上。通过循环伏安法（CV）评估了固定在金表面的这4种不同蛋白层的氧化还原特性。此外，通过计时电流法（CA）验证了它们的记忆功能。基于这些结果，我们证明了使用各种金属蛋白作为活性材料可以实现多位生物记忆概念。
