Protein-based multi-bit biomemory device consisting of various metalloproteins on self-assembled 11-MUA layer.

A multi-bit biomemory device was devised by introducing 4 different metalloproteins (azurin, cytochrome c, ferredoxin, myoglobin) to an electronic device using 11-MUA (11-mercapto-undecanoic acid) as the chemical linker. The immobilization of the 4 different self-assembled protein layers on a Au substrate via 11-MUA were confirmed by Raman spectroscopy and atomic force microscopy (AFM). The redox properties of these 4 different protein layers immobilized onto Au surface were assessed by cyclic voltammetry (CV). In addition, their memory functions were verified by chronoamperometry (CA). Based on these results, we demonstrated that a multi-bit biomemory concept could be realized using various metalloproteins as active materials.