Lim Sung-Hyuk, Yamaguchi Munehiro, Nishimura Okio, Mie Yasuhiro, Tamura Tomohiro, Kim Byung-Woo, Suzuki Masaaki
Advanced Industrial Science and Technology, (AIST), Sapporo 062-8517, Japan.
J Nanosci Nanotechnol. 2009 Dec;9(12):7318-23. doi: 10.1166/jnn.2009.1765.
We have succeeded to immobilize fluorescent proteins selectively using a micro-structured organosilane self-assembled monolayer as a template. An organosilane layer with amino terminal group was formed on a thermally oxidized Si wafer by liquid-phase method and then was pattern-etched by vacuum ultraviolet light (VUV). The second organosilane layer with thiol terminal group was deposited on the etched area by chemical vapor surface modification method (CVSM). These micro-structured organosilane layer containing two reactive terminal groups were chemically modified using bi-functional linkers. Two kinds of fluorescent protein, Enhanced Cyan Fluorescent Protein (ECFP) and R-phycoerythrin were selectively immobilized on the chemically modified surface.
我们已成功地以微结构化有机硅烷自组装单分子层为模板选择性地固定荧光蛋白。通过液相法在热氧化硅晶片上形成具有氨基端基的有机硅烷层，然后用真空紫外光（VUV）进行图案蚀刻。通过化学气相表面改性法（CVSM）在蚀刻区域沉积具有硫醇端基的第二有机硅烷层。使用双功能连接体对这些含有两个反应性端基的微结构化有机硅烷层进行化学修饰。两种荧光蛋白，即增强型青色荧光蛋白（ECFP）和R-藻红蛋白被选择性地固定在化学修饰的表面上。
