Advanced Industrial Science and Technology (AIST), 2-17-2-1, Tsukisamu-Higashi, Sapporo, 062-8517 Japan.
Langmuir. 2011 Oct 18;27(20):12521-32. doi: 10.1021/la202904g. Epub 2011 Sep 22.
Micropatterning techniques have become increasingly important in cellular biology. Cell patterning is achieved by various methods. Photolithography is one of the most popular methods, and several light sources (e.g., excimer lasers and mercury lamps) are used for that purpose. Vacuum ultraviolet (VUV) light that can be produced by an excimer lamp is advantageous for fabricating material patterns, since it can decompose organic materials directly and efficiently without photoresist or photosensitive materials. Despite the advantages, applications of VUV light to pattern biological materials are few. We have investigated cell patterning by using a template of a microstructured organosilane layer fabricated by VUV lithography. We first made a template of a microstructured organosilane layer by VUV lithography. Cell adhesive materials (poly(d-lysine) and polyethyleneimine) were chemically immobilized on the organosilane template, producing a cell adhesive material pattern. Primary rat cardiac and neuronal cells were successfully patterned by culturing them on the pattern substrate. Long-term culturing was attained for up to two weeks for cardiac cells and two months for cortex cells. We have discussed the reproducibility of cell patterning and made suggestions to improve it.
微图案化技术在细胞生物学中变得越来越重要。细胞图案化可通过各种方法实现。光刻是最常用的方法之一，为此目的使用了几种光源（例如准分子激光器和汞灯）。准分子灯产生的真空紫外（VUV）光有利于制造材料图案，因为它可以直接且有效地分解有机材料，而无需使用光致抗蚀剂或光敏材料。尽管有这些优势，但 VUV 光在生物材料图案化方面的应用却很少。我们已经通过使用由 VUV 光刻制成的微结构有机硅层模板来研究细胞图案化。我们首先通过 VUV 光刻制作了微结构有机硅层模板。细胞粘附材料（聚（D-赖氨酸）和聚乙烯亚胺）通过化学方法固定在有机硅模板上，从而产生细胞粘附材料图案。通过在图案化基底上培养，成功地对原代大鼠心脏和神经元细胞进行了图案化。心脏细胞的长期培养可长达两周，皮层细胞的培养可长达两个月。我们已经讨论了细胞图案化的可重复性，并提出了改进建议。
