Lehrstuhl fur Lebensmittelchemie der Technischen Universitat Munchen, Lichtenbergstrasse 4, D-85748 Garching, Germany.
Chem Res Toxicol. 2009 Dec;22(12):1929-37. doi: 10.1021/tx900236g.
The metabolism of the potent carcinogen estragole was investigated in humans after consumption of fennel tea by analyses of its metabolites in blood plasma and urine. Stable isotope dilution assays based on LC-MS/MS detection revealed that 1'-hydroxylation of estragole happened very fast as the concentration of conjugated 1'-hydroxyestragole in urine peaked after 1.5 h, whereas it was no longer detectable after 10 h. Besides the formation of less than 0.41% conjugated 1'-hydroxyestragole of the estragole dose administered, the further metabolite p-allylphenol was generated from estragole in a higher percentage (17%). Both metabolites were also detected in blood plasma in less than 0.75-2.5 h after consumption of fennel tea. In contrast to this, no estragole was present in these samples above its detection limit. From the results, it can be concluded that an excess of the major fennel odorant trans-anethole principally does not interfere with estragole metabolism, whereas influences on the quantitative composition of metabolites cannot be excluded. The presence of a sulfuric acid conjugate of estragole could not be confirmed, possibly due to its high reactivity and lability.
在人类摄入茴香茶后，通过分析其在血浆和尿液中的代谢物，研究了强致癌剂茴香脑的代谢情况。基于 LC-MS/MS 检测的稳定同位素稀释分析显示，茴香脑的 1'-羟化作用发生得非常快，因为尿液中结合的 1'-羟基茴香脑的浓度在 1.5 小时后达到峰值，而在 10 小时后则不再检测到。除了形成的共轭 1'-羟基茴香脑不到给予的茴香脑剂量的 0.41%之外，还从茴香脑中生成了更高比例（17%）的进一步代谢产物对丙烯基苯酚。这两种代谢物在摄入茴香茶后不到 0.75-2.5 小时也在血浆中检测到。相比之下，在这些样本中，茴香脑的含量均未超过检测限。从结果可以得出结论，主要的茴香气味物质反式茴香脑的过量摄入不会干扰茴香脑的代谢，而对代谢物的定量组成的影响则不能排除。由于其高反应性和不稳定性，不能确认存在茴香脑的硫酸结合物。
