Department of Transfusion, Huashan Hospital, Fudan University, Shanghai 200003, China.
Clin Biochem. 2010 Mar;43(4-5):397-400. doi: 10.1016/j.clinbiochem.2009.10.059. Epub 2009 Nov 10.
To investigate the levels of APRIL, BlyS and receptors as TACI, BCMA and BAFF-R in peripheral blood mononuclear cells (PBMC) of cryptococcal meningitis (CM) patients and its clinical significance.
PBMC from 30 CM patients and 32 healthy controls were isolated. The mRNA levels of APRIL, BLyS and BLyS receptors were detected by fluorescent quantitation PCR. The effect of PBMC from CM patients on in vitro growth of Cryptococcus neoformans was compared in presence and absence of BLyS.
PBMC of CM patients exhibited significantly lower BLyS, TACI and BCMA mRNA levels but significantly higher BAFF-R mRNA levels than controls. Growth of C. neoformans was significantly slower in presence of BLyS than its absence.
Levels of BlyS and its receptors correlated with cryptococcal meningitis progression, and provide new clues for monitoring CM conditions and its effective therapy.
研究抗蛋白聚糖（APRIL）、B 淋巴细胞刺激因子（BlyS）及其受体（TACI、BCMA 和 BAFF-R）在外周血单个核细胞（PBMC）中的水平及其临床意义。
分离 30 例隐球菌性脑膜炎（CM）患者和 32 例健康对照者的 PBMC，荧光定量 PCR 检测 APRIL、BlyS 和 BlyS 受体的 mRNA 水平。比较 CM 患者 PBMC 在存在和不存在 BlyS 时对新型隐球菌体外生长的影响。
CM 患者的 PBMC 中 BlyS、TACI 和 BCMA 的 mRNA 水平明显低于对照组，而 BAFF-R 的 mRNA 水平明显高于对照组。BlyS 存在时新型隐球菌的生长速度明显慢于 BlyS 不存在时。
BlyS 及其受体的水平与隐球菌性脑膜炎的进展相关，为监测 CM 病情和有效治疗提供了新的线索。
