Ju Shaoqing, Zhang Donglei, Wang Yueguo, Ni Hongbing, Kong Xiantao, Zhong Renqian
Department of Laboratory Diagnositics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Clin Biochem. 2006 Dec;39(12):1131-7. doi: 10.1016/j.clinbiochem.2006.09.010. Epub 2006 Oct 5.
To measure the levels of B-lymphocyte stimulator (BLyS) and its receptors mRNA expression in peripheral blood mononuclear cells (PBMCs) using quantitative real-time polymerase chain reaction (PCR) method and to investigate the relationship between BLyS and its receptors mRNA expression and systemic lupus erythematosus (SLE).
Specific primers and TaqMan probe were designed, and real-time PCR was performed. According to the standard curve of plasmids DNA, the levels of BLyS and its receptors mRNA expression in 37 patients with SLE and 30 healthy subjects were determined. The ratio of the expression levels of BLyS mRNA to that of beta2-microglobulin (beta2M) mRNA and the ratio of the expression levels of BLyS receptors mRNA to that of beta2M mRNA were regarded as indicator for the levels of BLyS and its receptors mRNA expression.
The expression of BLyS, TACI and BAFF-R mRNA in PBMCs from patients with SLE was significantly elevated compared to healthy controls (P<0.001 for each), and active patients with SLE group had higher mRNA expression than patients with SLE inactive group (P<0.001 for each). The patients with elevated anti-double-stranded DNA (anti-dsDNA) antibody titers had enhanced BLyS, TACI and BAFF-R mRNA expression (P<0.05 for BLyS; and P<0.01 for TACI and BAFF-R).
The BLyS, TACI and BAFF-R mRNA expression levels were significantly elevated in patients with SLE, which suggests that BLyS, TACI and BAFF-R might be involved in the pathogenesis, and that mRNA expression levels might serve as a biomarker of disease activity.
采用定量实时聚合酶链反应（PCR）方法检测外周血单个核细胞（PBMCs）中B淋巴细胞刺激因子（BLyS）及其受体mRNA的表达水平，并探讨BLyS及其受体mRNA表达与系统性红斑狼疮（SLE）之间的关系。
设计特异性引物和TaqMan探针，进行实时PCR。根据质粒DNA标准曲线，测定37例SLE患者和30例健康对照者外周血中BLyS及其受体mRNA的表达水平。将BLyS mRNA表达水平与β2-微球蛋白（β2M）mRNA表达水平的比值以及BLyS受体mRNA表达水平与β2M mRNA表达水平的比值作为BLyS及其受体mRNA表达水平的指标。
与健康对照相比，SLE患者PBMCs中BLyS、跨膜激活剂和钙调亲环素配体相互作用分子（TACI）及B淋巴细胞刺激因子受体（BAFF-R）mRNA的表达显著升高（各P<0.001），SLE活动期患者组mRNA表达高于SLE非活动期患者组（各P<0.001）。抗双链DNA（抗dsDNA）抗体滴度升高的患者，其BLyS、TACI及BAFF-R mRNA表达增强（BLyS，P<0.05；TACI和BAFF-R，P<0.01）。
SLE患者BLyS、TACI及BAFF-R mRNA表达水平显著升高，提示BLyS、TACI及BAFF-R可能参与SLE的发病机制，且mRNA表达水平可能作为疾病活动的生物标志物。
