应用免疫印迹技术研究犬小孢子菌抗原蛋白在犬皮肤真菌病中的作用。

Use of western blot to study Microsporum canis antigenic proteins in canine dermatophytosis.

机构信息

Department of Animal Productions, Epidemiology and Ecology, Veterinary Faculty, University of Turin, Turin, Italy.

出版信息

Mycoses. 2011 May;54(3):223-9. doi: 10.1111/j.1439-0507.2009.01807.x.

DOI:10.1111/j.1439-0507.2009.01807.x

PMID:19912544

Abstract

Western blotting was used to describe the Microsporum canis proteins with antigenic activity in dogs with dermatophytosis. Electrophoretic separation of whole fungal strain extract cultured from a cat was performed under denaturing conditions. The proteins were blotted onto nitrocellulose and probed with sera collected from 22 dogs with dermatophytosis (18 M. canis, 3 M. gypseum, 1 Trichophyton mentagrophytes; group A), 20 dogs with skin diseases other than dermatophytosis, and 22 dogs with no clinical cutaneous signs (group B, n = 42). Nine principal IgG-binding proteins with apparent molecular weights of 180, 144, 130, 120, 102, 96, 80, 68, and 48 kD were visualised on group A blots. For these proteins, serological cross-reactivity with different strains of M. canis may be indirectly confirmed, whereas additional proteins were found to react with sera from individual dogs. The proteins visualised in this study may represent diagnostic markers of dermatophyte infection. The proteins should be further evaluated for their role in the cellular immune response of dogs with dermatophytosis.

摘要

免疫印迹法用于描述患有皮肤真菌病的犬的犬小孢子菌具有抗原活性的蛋白质。在变性条件下对从猫身上培养的整个真菌菌株提取物进行电泳分离。将蛋白质印迹转移到硝酸纤维素上，并与来自 22 只患有皮肤真菌病（18 只为犬小孢子菌、3 只为石膏样小孢子菌、1 只为须毛癣菌；A 组）、20 只患有非皮肤真菌病的狗和 22 只无临床皮肤症状的狗（B 组，n = 42）的血清进行探测。在 A 组印迹上可以看到 9 种主要的 IgG 结合蛋白，其表观分子量分别为 180、144、130、120、102、96、80、68 和 48 kD。对于这些蛋白质，可以间接证实与不同株犬小孢子菌的血清学交叉反应性，而其他蛋白质则与个别狗的血清发生反应。本研究中观察到的蛋白质可能代表皮肤真菌病感染的诊断标志物。应进一步评估这些蛋白质在患有皮肤真菌病的犬的细胞免疫反应中的作用。