Department of Veterinary Anatomy and Neuroscience Program, Iowa State University, Ames, Iowa 50011.
Mol Cell Neurosci. 1993 Aug;4(4):354-65. doi: 10.1006/mcne.1993.1045.
Galanin (GAL) is a 29-amino acid peptide widely distributed in the mammalian nervous system. Recently, GAL expression has been shown to increase during periods of neuronal degeneration (e.g., Alzheimer's disease) and neuronal injury. Our laboratory has previously reported that GAL immunoreactivity (GAL-IR) is robustly expressed during a time of CNS plasticity, development. The striking GAL-IR seen during times of CNS morphogenesis and neurogenesis led us to the hypothesis that GAL may play a role in the formation of the nervous system. To further substantiate a developmental role for GAL we have used autoradiography to define the distribution of GAL receptors in the forming mammalian brain. To this end, we have used the Brazilian opossum, Monodelphis domestica, as a developmental model. In this study, we have described the profile of GAL receptors in the developing mammalian brain. [(125)I]GAL binding was detected as early as 1 day of postnatal life in regions of the brain which were still undergoing neurogenesis. High levels of GAL receptor expression were region specific and correlated with our previous results on GAL-IR during development. In addition, a transient binding pattern was seen in the anterior pituitary. In the adult brain, the pattern seen was very similar to that of reports in other species. Due to the observations of the presence of GAL and its receptor during times of active neurogenesis and morphogenesis we believe galanin may play an important role in morphogenesis and early functioning of the mammalian CNS.
甘丙肽（GAL）是一种广泛分布于哺乳动物神经系统的 29 个氨基酸肽。最近的研究表明，GAL 的表达在神经元退化（如阿尔茨海默病）和神经元损伤期间增加。我们实验室之前的研究报告称，GAL 免疫反应性（GAL-IR）在中枢神经系统可塑性和发育期间强烈表达。在中枢神经系统形态发生和神经发生期间观察到的显著 GAL-IR 促使我们假设 GAL 可能在神经系统的形成中发挥作用。为了进一步证实 GAL 在发育中的作用，我们使用放射自显影术来确定形成中的哺乳动物大脑中 GAL 受体的分布。为此，我们使用了有袋动物巴西负鼠（Monodelphis domestica）作为发育模型。在这项研究中，我们描述了发育中的哺乳动物大脑中 GAL 受体的分布情况。早在出生后 1 天，在仍处于神经发生的大脑区域中就检测到了[(125)I]GAL 结合。高水平的 GAL 受体表达具有区域特异性，并与我们之前在发育过程中关于 GAL-IR 的结果相关。此外，在前垂体中观察到了短暂的结合模式。在成年大脑中，所见的模式与其他物种的报道非常相似。由于在活跃的神经发生和形态发生期间观察到 GAL 及其受体的存在，我们认为甘丙肽可能在哺乳动物中枢神经系统的形态发生和早期功能中发挥重要作用。
