Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry in Brno, Zemedelská 1, CZ-61300 Brno, Czech Republic.
J Chromatogr A. 2009 Dec 18;1216(51):9022-6. doi: 10.1016/j.chroma.2009.10.067. Epub 2009 Oct 30.
Polyelectrolyte multilayers deposited on the wall of fused silica capillaries were used as stationary phases in open tubular ion chromatography. The multilayers were formed by flushing the capillaries with solutions of polyanions and polycations such as polydiallyldimethylammonium chloride and dextran sulphate. Columns with several bi-layers were constructed and used in low pressure non-suppressed open tubular ion chromatography of common inorganic anions (F(-), Cl(-), NO(3)(-)) and cations (Li(+), Na(+), NH(4)(+), K(+), Cs(+)) with contactless conductometric detection. Using sodium benzoate and tartaric acid eluents the separations were typically achieved in less than 35 min with separation efficiencies between 2000 and 9000 theoretical plates. A bi-functional column was prepared that contains both anionic and cationic functional groups and was used for simultaneous separation of anions and cations.
聚电解质多层膜沉积在熔融石英毛细管的壁上，用作开管离子色谱中的固定相。通过用聚阴离子和聚阳离子（如聚二烯丙基二甲基氯化铵和葡聚糖硫酸盐）溶液冲洗毛细管来形成多层膜。构建了具有几个双层的柱子，并用于低压非抑制型开管离子色谱法对常见无机阴离子（F(-)、Cl(-)、NO(3)(-)）和阳离子（Li(+)、Na(+)、NH(4)(+)、K(+)、Cs(+)）进行检测，采用非接触电导检测。使用苯甲酸钠和酒石酸洗脱剂，通常可以在不到 35 分钟的时间内实现分离，分离效率在 2000 到 9000 个理论塔板之间。制备了一种双功能柱，其中包含阴离子和阳离子官能团，用于同时分离阴离子和阳离子。
