Croy B A, Reed N, Malashenko B A, Kim K, Kwon B S
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Canada.
Cell Immunol. 1991 Mar;133(1):116-26. doi: 10.1016/0008-8749(91)90184-d.
Granulated metrial gland (GMG) cells are a consistently observed but poorly understood feature of the murine uterus during successful pregnancy. From morphological studies and antibody phenotyping it has been suggested that GMG cells may be members of the natural killer (NK) cell lineage. However, lysis of murine NK cell targets by GMG cells has not been observed although lysis of freshly dissociated trophoblast cells by GMG cells has been recorded using timelapse video. We failed to demonstrate significant interactions between migrating GMG cells, collected from explant cultures under previously reported cultures conditions, and YAC target cells. However, YAC cell lysis did occur if hrIL-2 was present throughout the periods of explant culture and lysis assay. Furthermore, lysis was enhanced if the pregnant females were treated with the interferon inducer poly I.C. 24 hr before metrial gland collection. GMG cells expressed perforin and serine protease mRNA. Consistent with the lysis experiments, expression of these genes was enhanced when the cells were incubated with hrIL-2. Our data provide further support for a relationship between GMG cells and NK cells, but do not establish a relationship of identity since hrIL-2, a growth factor sufficient for the culture of NK cells, cannot support growth or prolong survival of GMG cells.
颗粒状蜕膜腺（GMG）细胞是成功妊娠期间小鼠子宫中一直被观察到但了解甚少的一个特征。从形态学研究和抗体表型分析来看，有人提出GMG细胞可能是自然杀伤（NK）细胞谱系的成员。然而，虽然通过延时视频记录发现GMG细胞可裂解新鲜解离的滋养层细胞，但未观察到GMG细胞对小鼠NK细胞靶标的裂解作用。我们未能证明在先前报道的培养条件下从外植体培养物中收集的迁移性GMG细胞与YAC靶细胞之间存在显著相互作用。然而，如果在整个外植体培养和裂解测定期间都存在人重组白细胞介素-2（hrIL-2），则确实会发生YAC细胞裂解。此外，如果在收集蜕膜腺前24小时用干扰素诱导剂聚肌胞苷酸（poly I.C.）处理妊娠雌性小鼠，裂解作用会增强。GMG细胞表达穿孔素和丝氨酸蛋白酶mRNA。与裂解实验一致，当细胞与hrIL-2一起孵育时，这些基因的表达会增强。我们的数据进一步支持了GMG细胞与NK细胞之间的关系，但并未确立它们的同一性，因为hrIL-2作为一种足以培养NK细胞的生长因子，无法支持GMG细胞的生长或延长其存活时间。
