Ampel N M, Galgiani J N
Medical Service, Tucson Veterans Administration Medical Center, Arizona.
Cell Immunol. 1991 Mar;133(1):253-62. doi: 10.1016/0008-8749(91)90195-h.
We explored the in vitro interaction of human peripheral blood mononuclear cells with the arthroconidial stage of the fungus Coccidioides immitis. Fresh peripheral blood monocytes in an adherent monolayer were capable of ingesting C. immitis. Further, peripheral blood monocytes from either skin-test-positive or skin-test-negative donors significantly decreased the in vitro growth of C. immitis when coccidioidal arthroconidia were incubated with monocytes. Peripheral blood mononuclear cells also reduced fungal incorporation of the chitin precursor N-acetyl glucosamine. Cell fractions consisting predominantly of monocytes were significantly more active in this regard than fractions containing predominantly lymphocytes. Moreover, this activity was independent of the coccidioidal skin-test status of the donor. We conclude that human fresh peripheral blood mononuclear cells are able to phagocytize C. immitis arthroconidia and have the ability to inhibit its growth in vitro. That these abilities are independent of the immune status of the donor supports the possibility that the peripheral blood monocyte may contribute to the early defense against initial coccidioidal infection.
我们研究了人类外周血单核细胞与真菌粗球孢子菌关节孢子阶段的体外相互作用。贴壁单层培养的新鲜外周血单核细胞能够摄取粗球孢子菌。此外，当将球孢子菌关节孢子与单核细胞共同孵育时，来自皮肤试验阳性或皮肤试验阴性供体的外周血单核细胞均能显著降低粗球孢子菌的体外生长。外周血单核细胞还减少了几丁质前体N-乙酰葡糖胺的真菌掺入。主要由单核细胞组成的细胞组分在这方面的活性明显高于主要由淋巴细胞组成的组分。此外，这种活性与供体的球孢子菌皮肤试验状态无关。我们得出结论，人类新鲜外周血单核细胞能够吞噬粗球孢子菌关节孢子，并具有在体外抑制其生长的能力。这些能力与供体的免疫状态无关，这支持了外周血单核细胞可能有助于对初始球孢子菌感染进行早期防御的可能性。
