Galgiani J N, Payne C M, Jones J F
J Infect Dis. 1984 Mar;149(3):404-12. doi: 10.1093/infdis/149.3.404.
Human polymorphonuclear leukocytes (PMNLs), when added to arthroconidial suspensions of Coccidioides immitis, markedly inhibit fungal incorporation of the cell-wall precursor N-acetylglucosamine. This effect does not require serum but is facilitated by a heat-labile serum component(s), probably by promoting PMNL attachment to the arthroconidia. Inhibition is entirely reversible within 24 hr. In parallel with this finding, PMNLs exhibited virtually no killing of arthroconidia and (as determined by electron microscopy) did not produce damage to the fungal ultrastructure. PMNLs from a patient with chronic granulomatous disease suppressed fungal incorporation of N-acetyl-glucosamine by only 0%-22% under various conditions, as compared with 54%-85% suppression by PMNLs from normal donors. Our studies demonstrate that PMNLs can influence the metabolism of C immitis and raise the possibility that their presence in coccidioidal histopathology may be an important immediate host defense in limiting progression early after infection.
当将人类多形核白细胞（PMNLs）添加到粗球孢子菌的关节孢子悬液中时，可显著抑制真菌对细胞壁前体N - 乙酰葡糖胺的摄取。这种效应不需要血清，但可由一种热不稳定的血清成分促进，可能是通过促进PMNLs附着于关节孢子来实现。在24小时内抑制作用完全可逆。与此发现并行的是，PMNLs几乎没有杀死关节孢子，并且（通过电子显微镜确定）没有对真菌超微结构造成损伤。与正常供体的PMNLs抑制率为54% - 85%相比，慢性肉芽肿病患者的PMNLs在各种条件下对N - 乙酰葡糖胺的真菌摄取抑制率仅为0% - 22%。我们的研究表明，PMNLs可影响粗球孢子菌的代谢，并增加了其在球孢子菌组织病理学中的存在可能是感染后早期限制病情进展的重要即时宿主防御的可能性。
