Beaman L
Medical Microbiology and Immunology, University of California School of Medicine, Davis 95616.
Infect Immun. 1991 Nov;59(11):4227-9. doi: 10.1128/iai.59.11.4227-4229.1991.
Human peripheral blood monocytes readily phagocytized Coccidioides immitis endospores (2 to 5 microns) in vitro. Within 24 to 30 h at 37 degrees C, the phagocytized endospores started developing into immature spherules. However, when the monocytes were incubated with recombinant human gamma interferon (rIFN-gamma) or recombinant human tumor necrosis factor alpha (rTNF-alpha) and then infected, fewer endospores developed into spherules. Treatment with rIFN-gamma or rTNF-alpha activated the fungicidal capabilities of the monocytes as evidenced by the significant reduction in CFU that could be recovered from rIFN-gamma- or rTNF-alpha-activated monocytes compared with nontreated controls.
人外周血单核细胞在体外能轻易吞噬粗球孢子菌内孢子（2至5微米）。在37摄氏度下24至30小时内，被吞噬的内孢子开始发育成未成熟的球形体。然而，当单核细胞与重组人γ干扰素（rIFN-γ）或重组人肿瘤坏死因子α（rTNF-α）一起孵育然后感染时，发育成球形体的内孢子较少。与未处理的对照相比，用rIFN-γ或rTNF-α处理激活了单核细胞的杀真菌能力，这可从rIFN-γ或rTNF-α激活的单核细胞中回收的菌落形成单位（CFU）显著减少得到证明。
