Institut für Pharmazeutische Chemie, Philipps-Universität Marburg, Germany.
Biosens Bioelectron. 2010 Jan 15;25(5):967-71. doi: 10.1016/j.bios.2009.04.002. Epub 2009 Apr 14.
An SPR-based sandwich immunoassay for serotyping of Salmonella is demonstrated. The Salmonella are captured on an SPR chip using polyclonal capture antibody. SPR sensorgrams are obtained for the immunoreactions of the somatic (O) and flagellar (H) surface antigens, of the captured bacteria, to their respective antibodies. The sensorgram data are compiled to determine the antigenic formula in accordance with the Kauffmann-White scheme. Salmonella Enteritidis was completely serotyped using this SPR-based method. In addition, Salmonella belonging to serogroups B, C and D were successfully assigned to their respective serogroups. Before serotyping the bacteria are grown to a concentration of 1x10(10) mL(-1). This SPR-based serotyping provides quantitative data, and thus, eliminates the possibility of false detections as encountered in the conventional slide agglutination test (SAT). This method was also proved to work with rough strains.
建立了基于表面等离子体共振（SPR）的沙门氏菌血清分型夹心免疫测定法。用多克隆捕获抗体将沙门氏菌捕获在 SPR 芯片上。对捕获细菌的菌体（O）和鞭毛（H）表面抗原与相应抗体的免疫反应进行 SPR 传感器图记录。根据 Kauffmann-White 方案，将传感器图数据进行编译，以确定抗原公式。使用这种基于 SPR 的方法可以对肠炎沙门氏菌进行完全的血清分型。此外，成功地将属于 B、C 和 D 血清群的沙门氏菌分配到各自的血清群。在血清分型之前，将细菌培养到浓度为 1x10(10) mL(-1)。这种基于 SPR 的血清分型提供定量数据，从而消除了传统玻片凝集试验（SAT）中遇到的假检测的可能性。该方法也被证明适用于粗糙型菌株。
