Experimental Zooprophylactic Institute of Apulia and Basilicata, Foggia, Italy.
Food Microbiol. 2010 Feb;27(1):101-8. doi: 10.1016/j.fm.2009.09.001. Epub 2009 Sep 10.
Standardized tools for typing Listeria monocytogenes isolates are required in epidemiological surveys investigating food-borne disease outbreaks and in the food-processing environment to identify the sources of contamination and routes by which the organisms are spread. In this survey Amplified Fragment Length Polymorphism (AFLP) and Multi-Locus Sequence Typing (MLST) have been used to study 103 L. monocytogenes isolates from food and environmental sources. A total of 62 AFLP types and 66 MLST Sequence Types were identified. AFLP and MLST produced similar results in terms of discriminating power. The Discrimination Index calculated for the two techniques was 0.976 for AFLP and 0.972 for MLST. These values were appreciably higher compared to serotyping (0.739). A good congruence was observed between AFLP and MLST. The present study demonstrated that AFLP and MLST subtyping are suitable tools for studying the epidemiology of L. monocytogenes. The great advantage of MLST over AFLP and other molecular typing methods based on fragment fingerprinting lies in the unambiguity of sequence data while AFLP is less costly and highly processive. In conclusion the two methods can be perfectly integrated for high-resolution genotyping of L. monocytogenes.
在调查食源性疾病暴发和食品加工环境的流行病学调查中，需要标准化的工具来对李斯特菌进行分型，以确定污染的来源和传播途径。在这项调查中，扩增片段长度多态性（AFLP）和多位点序列分型（MLST）被用于研究来自食品和环境来源的 103 株李斯特菌。共鉴定出 62 种 AFLP 型和 66 种 MLST 序列型。AFLP 和 MLST 在区分能力方面产生了相似的结果。两种技术的鉴别指数分别为 AFLP 的 0.976 和 MLST 的 0.972，与血清分型（0.739）相比，这些值明显更高。AFLP 和 MLST 之间存在良好的一致性。本研究表明，AFLP 和 MLST 分型是研究李斯特菌流行病学的合适工具。MLST 相对于 AFLP 和其他基于片段指纹图谱的分子分型方法的巨大优势在于序列数据的明确性，而 AFLP 则成本更低且高度连续。总之，这两种方法可以完美地结合起来，对李斯特菌进行高分辨率的基因分型。
