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基于生物启发从[植物名称]的生长嫩枝中简便制备银纳米颗粒：阐述其在抑制[细菌名称]生长和生物膜形成方面的潜力以及评估伤口愈合能力。

Bio-inspired facile fabrication of silver nanoparticles from grown shoots of : explication of its potential in impeding growth and biofilms of and assessment of wound healing ability.

作者信息

Al-Shabib Nasser A, Husain Fohad Mabood, Nadeem Mohammad, Khan Mohd Shahnawaz, Al-Qurainy Fahad, Alyousef Abdullah A, Arshad Mohammed, Khan Altaf, Khan Javed Masood, Alam Pravej, Albalawi Thamer, Shahzad Syed Ali

机构信息

Department of Food Science and Nutrition, Faculty of Food and Agricultural Sciences, King Saud University 2456 Riyadh 11451 Kingdom of Saudi Arabia

Department of Botany and Microbiology, College of Science, King Saud University 2456 Riyadh 11451 Kingdom of Saudi Arabia.

出版信息

RSC Adv. 2020 Aug 17;10(50):30139-30149. doi: 10.1039/d0ra04587j. eCollection 2020 Aug 10.

DOI:10.1039/d0ra04587j
PMID:35518236
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9056294/
Abstract

Novel, safe, and effective antilisterial agents are required in order to prevent infections and maintain food safety. This study synthesized silver nanoparticles (AgNPs) from the shoot extract of -grown (TN) and characterized them using X-ray diffraction, Fourier transform infrared spectroscopy, UV-visible spectroscopy, dynamic light scattering, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDXS), and transmission electron microscopy (TEM). We also assessed the antilisterial potential of the synthesized TN-AgNPs by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against two strains of and . TN-AgNPs (2×MICs) showed a significant decrease in growth in all test strains. Release of cellular content and cell morphology analysis of TN-AgNP-treated bacterial cells demonstrated the mechanism of bactericidal activity of AgNPs. In addition, TN-AgNPs induced a significant decrease in swimming motility (62-71%), biofilm formation (57-64%), and preformed biofilms (48-58%) in all test strains at sub-inhibitory concentrations. Microtitre plate assay results for biofilm inhibition were confirmed by SEM and CLSM visualization of TN-AgNP-treated and TN-AgNP-untreated test strains. TN-AgNPs also showed wound-healing activity in MCF-7 cells by inhibiting cell migration in a scratch plate assay. TN-AgNP-induced enhanced reactive oxygen species generation in treated cells could be a plausible reason for the biofilm inhibitory activity of AgNPs. TN-AgNPs having antilisterial, antibiofilm, and wound-healing properties can be effectively used to prevent infections in the food industry and healthcare.

摘要

为了预防感染并维持食品安全，需要新型、安全且有效的抗李斯特菌剂。本研究从生长的（TN）茎提取物中合成了银纳米颗粒（AgNPs），并使用X射线衍射、傅里叶变换红外光谱、紫外可见光谱、动态光散射、扫描电子显微镜（SEM）、能量色散X射线光谱（EDXS）和透射电子显微镜（TEM）对其进行了表征。我们还通过测定对两株和的最小抑菌浓度（MIC）和最小杀菌浓度（MBC），评估了合成的TN-AgNPs的抗李斯特菌潜力。TN-AgNPs（2×MICs）在所有测试菌株中均显示出显著的生长抑制。对TN-AgNP处理的细菌细胞进行细胞内容物释放和细胞形态分析，揭示了AgNPs的杀菌活性机制。此外，TN-AgNPs在亚抑菌浓度下，使所有测试菌株的游泳运动性（62-71%）、生物膜形成（57-64%）和预先形成的生物膜（48-58%）显著降低。通过SEM和CLSM对TN-AgNP处理和未处理的测试菌株进行可视化，证实了微量滴定板法对生物膜抑制的结果。TN-AgNPs在划痕平板试验中通过抑制细胞迁移，还显示出对MCF-7细胞的伤口愈合活性。TN-AgNP诱导处理细胞中活性氧生成增强，可能是AgNPs生物膜抑制活性的一个合理原因。具有抗李斯特菌、抗生物膜和伤口愈合特性的TN-AgNPs可有效用于预防食品工业和医疗保健中的感染。

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