根管封闭剂对哺乳动物细胞的细胞毒性、氧化应激及遗传毒性诱导作用
The induction of cytotoxicity, oxidative stress, and genotoxicity by root canal sealers in mammalian cells.
作者信息
Camargo Carlos Henrique Ribeiro, Camargo Samira Esteves Afonso, Valera Marcia Carneiro, Hiller Karl-Anton, Schmalz Gottfried, Schweikl Helmut
机构信息
Departament of Restorative Dentistry and Endodontics, São Paulo State University, São José dos Campos, Brazil.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Dec;108(6):952-60. doi: 10.1016/j.tripleo.2009.07.015.
OBJECTIVE
Toxicologic aspects of the root canal sealers Acroseal, Epiphany, AH Plus, and castor oil polymer (COP) were analyzed using cell culture techniques.
STUDY DESIGN
Set materials were extracted in culture medium, and cytotoxicity and the generation of reactive oxygen species (ROS) were determined in human pulp cells. The formation of micronuclei (MN), indicative of genotoxicity and changes of the cell cycle, were analyzed in V79 cells.
RESULTS
The ranking of the most to the least toxic material was: Acroseal > Epiphany = AH Plus >> COP. The production of ROS was increased by Epiphany, Acroseal, and AH Plus about 4-7-fold, but enhanced ROS production was not detected with COP. Acroseal, Epiphany, and AH Plus increased the numbers of MN, and Acroseal delayed the cell cycle in G2 phase.
CONCLUSION
The materials which tested cytotoxic in the present study may be able to alter oral tissue-specific functions and cause inflammation.
目的
采用细胞培养技术分析根管封闭剂Acroseal、Epiphany、AH Plus和蓖麻油聚合物（COP）的毒理学特性。
研究设计
将设定材料在培养基中提取，然后在人牙髓细胞中测定细胞毒性和活性氧（ROS）的产生情况。在V79细胞中分析微核（MN）的形成情况，微核形成表明存在遗传毒性和细胞周期变化。
结果
毒性由高到低的材料排序为：Acroseal > Epiphany = AH Plus >> COP。Epiphany、Acroseal和AH Plus使ROS的产生增加了约4 - 7倍，但未检测到COP会增强ROS的产生。Acroseal、Epiphany和AH Plus增加了MN的数量，且Acroseal使细胞周期在G2期延迟。
结论
在本研究中检测出具有细胞毒性的材料可能会改变口腔组织的特定功能并引发炎症。
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