比较一种快速免疫分析方法与传统方法检测欧洲犬类伯氏疏螺旋体 C6 抗原抗体。

Comparison of a rapid immunoassay for antibodies to the C6 antigen with conventional tests for antibodies to Borrelia burgdorferi in dogs in Europe.

机构信息

Clinic for Small Animal Internal Medicine, University of Zurich, Switzerland.

出版信息

Vet Rec. 2009 Nov 14;165(20):594-7. doi: 10.1136/vr.165.20.594.

DOI:10.1136/vr.165.20.594

PMID:19915191

Abstract

A commercial immunoassay for antibodies to the C6 antigen of Borrelia burgdorferi was evaluated against an IgG in-house ELISA in combination with a Western blot assay to examine 104 samples of serum from 53 healthy Bernese mountain dogs, which were suspected to have a breed predisposition to Lyme borreliosis, and 55 samples from 30 healthy large-breed longhair dogs. The two test methods correlated in 125 (79 per cent) of the samples with an agreement of kappa=0.571 (P<0.001). In comparison with the in-house ELISA in combination with a Western blot, the sensitivity and specificity of the C6 test were 81 per cent and 77 per cent respectively. The agreement between the tests was better with the samples from the Bernese mountain dogs (k=0.681) than with the samples from the control dogs (k=0.347).

摘要

一项针对伯氏疏螺旋体 C6 抗原的商业免疫分析试剂盒，与 IgG 酶联免疫吸附试验（ELISA）和免疫印迹法联合检测了 104 份来自 53 只疑似具有莱姆病遗传易感性的瑞士伯恩山犬和 55 份来自 30 只健康大型长毛犬的血清样本。两种检测方法在 125 份样本（79%）中具有相关性，kappa 值为 0.571（P<0.001）。与 IgG 酶联免疫吸附试验（ELISA）和免疫印迹法联合检测相比，C6 检测的敏感性和特异性分别为 81%和 77%。与对照组犬（k=0.347）相比，伯尔尼山犬（k=0.681）样本的检测结果一致性更好。