Kishimoto Yuji, Kano Rui, Maruyama Haruhiko, Onozaki Masanobu, Makimura Koichi, Ito Takaaki, Matsubara Kiyoshi, Hasegawa Atsuhiko, Kamata Hiroshi
Department of Pathobiology, Nihon University School of Veterinary Medicine, Japan.
J Vet Med Sci. 2010 Jan;72(1):123-6. doi: 10.1292/jvms.09-0115. Epub 2009 Nov 13.
The study has been conducted to assess the applicability of partial 26S ribosomal DNA sequence for the phylogenic analysis of P.zopfii and to arrive relationships between specific genotype of P.zopfii and clinical mastitis. The phylogenetic analysis indicated that all genotype 2 isolates were grouped into the cluster of type strain of P.zopfii. Thus, all mastitis isolates in Japan were identified as P.zopfii genotype 2 and were independent of the cluster of the genotype 1 isolates. In one clinical case, a fecal isolate could not be identified by the 18S rDNA-based genotype specific PCR-assay and appeared to belong to a same cluster with the type strain of P. blaschkeae by 26S rDNA analysis. The isolate was the first isolation in Japan.
本研究旨在评估部分26S核糖体DNA序列在毕赤酵母系统发育分析中的适用性，并确定毕赤酵母特定基因型与临床乳腺炎之间的关系。系统发育分析表明，所有基因型2的分离株都被归入毕赤酵母模式菌株的簇中。因此，日本所有乳腺炎分离株均被鉴定为毕赤酵母基因型2，且独立于基因型1分离株的簇。在一个临床病例中，基于18S rDNA的基因型特异性PCR检测无法鉴定粪便分离株，通过26S rDNA分析，该分离株似乎与布拉氏毕赤酵母模式菌株属于同一簇。该分离株是日本的首次分离。
