Irrgang A, Weise C, Murugaiyan J, Roesler U
Institute of Animal Hygiene and Environmental Health, Centre for Infection Medicine, Germany.
Institute for Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany.
New Microbes New Infect. 2014 Nov 8;3:37-40. doi: 10.1016/j.nmni.2014.10.004. eCollection 2015 Jan.
Prototheca zopfii associated with bovine mastitis and human protothecosis exists as two genotypes, of which genotype 1 is considered as non-infectious and genotype 2 as infectious. The mechanism of infection has not yet been described. The present study was aimed to identify genotype 2-specific immunodominant proteins. Prototheca proteins were separated using two-dimensional gel electrophoresis. Subsequent western blotting with rabbit hyperimmune serum revealed 28 protein spots. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis resulted in the identification of 15 proteins including malate dehydrogenase, elongation factor 1-alpha, heat shock protein 70, and 14-3-3 protein, which were previously described as immunogenic proteins of other eukaryotic pathogens.
与牛乳腺炎和人类原虫病相关的佐夫原虫存在两种基因型，其中基因型1被认为是非感染性的，基因型2是感染性的。感染机制尚未得到描述。本研究旨在鉴定基因型2特异性免疫显性蛋白。使用二维凝胶电泳分离原虫蛋白。随后用兔超免疫血清进行蛋白质印迹分析，显示出28个蛋白斑点。基质辅助激光解吸电离飞行时间质谱分析鉴定出15种蛋白质，包括苹果酸脱氢酶、延伸因子1-α、热休克蛋白70和14-3-3蛋白，这些蛋白先前被描述为其他真核病原体的免疫原性蛋白。
