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利用成像质谱法对代谢物交换进行翻译。

Translating metabolic exchange with imaging mass spectrometry.

作者信息

Yang Yu-Liang, Xu Yuquan, Straight Paul, Dorrestein Pieter C

机构信息

University of California, San Diego, La Jolla, USA.

出版信息

Nat Chem Biol. 2009 Dec;5(12):885-7. doi: 10.1038/nchembio.252. Epub 2009 Nov 8.

DOI:10.1038/nchembio.252
PMID:19915536
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2778862/
Abstract

Metabolic exchange between an organism and the environment, including interactions with neighboring organisms, is important for processes of organismal development. Here we develop and use thin-layer agar natural product MALDI-TOF imaging mass spectrometry of intact bacterial colonies grown on top of the MALDI target plate to study an interaction between two species of bacteria and provide direct evidence that Bacillus subtilis silences the defensive arsenal of Streptomyces coelicolor.

摘要

生物体与环境之间的代谢交换，包括与邻近生物体的相互作用，对于生物体发育过程很重要。在这里，我们开发并使用在基质辅助激光解吸/电离（MALDI）靶板顶部生长的完整细菌菌落的薄层琼脂天然产物MALDI-TOF成像质谱来研究两种细菌之间的相互作用，并提供直接证据表明枯草芽孢杆菌使天蓝色链霉菌的防御武器库沉默。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dae7/2778862/3decd6be041b/nihms145195f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dae7/2778862/712c869f3062/nihms145195f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dae7/2778862/3ca535b32e3d/nihms145195f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dae7/2778862/3decd6be041b/nihms145195f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dae7/2778862/712c869f3062/nihms145195f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dae7/2778862/3ca535b32e3d/nihms145195f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dae7/2778862/3decd6be041b/nihms145195f3.jpg

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