利用薄膜解吸电喷雾电离成像质谱技术捕获细菌代谢交换。
Capturing bacterial metabolic exchange using thin film desorption electrospray ionization-imaging mass spectrometry.
出版信息
Anal Chem. 2010 Mar 1;82(5):1598-600. doi: 10.1021/ac9027388.
Abstract
Over 60% of current pharmaceutical drugs have origins in natural products. To expand on current methods allowing one to characterize natural products directly from bacterial culture, herein we describe the use of desorption electrospray ionization (DESI) imaging mass spectrometry in monitoring the exchange of secondary metabolites between Bacillus subtilis and Streptomyces coelicolor using a simple imprinting technique.
摘要
超过 60%的现有药物都源自天然产物。为了扩展当前可直接从细菌培养物中对天然产物进行特征分析的方法，本研究采用解吸电喷雾电离（DESI）成像质谱技术，结合简单的印迹技术，监测枯草芽孢杆菌和变铅青链霉菌之间次生代谢物的交换。
相似文献
1
Capturing bacterial metabolic exchange using thin film desorption electrospray ionization-imaging mass spectrometry.利用薄膜解吸电喷雾电离成像质谱技术捕获细菌代谢交换。
Anal Chem. 2010 Mar 1;82(5):1598-600. doi: 10.1021/ac9027388.
2
Metabolic profiling directly from the Petri dish using nanospray desorption electrospray ionization imaging mass spectrometry.采用纳喷雾解吸电喷雾电离成像质谱法直接从培养皿中进行代谢轮廓分析。
Anal Chem. 2013 Nov 5;85(21):10385-91. doi: 10.1021/ac4023154. Epub 2013 Oct 14.
3
Translating metabolic exchange with imaging mass spectrometry.利用成像质谱法对代谢物交换进行翻译。
Nat Chem Biol. 2009 Dec;5(12):885-7. doi: 10.1038/nchembio.252. Epub 2009 Nov 8.
4
Characterization and mapping of secondary metabolites of Streptomyces sp. from caatinga by desorption electrospray ionization mass spectrometry (DESI-MS).利用解吸电喷雾电离质谱法（DESI-MS）对来自卡廷加的链霉菌属的次生代谢产物进行表征和定位。
Anal Bioanal Chem. 2018 Nov;410(27):7135-7144. doi: 10.1007/s00216-018-1315-0. Epub 2018 Sep 8.
5
In vivo recognition of Bacillus subtilis by desorption electrospray ionization mass spectrometry (DESI-MS).体内鉴定枯草芽孢杆菌的解吸电喷雾电离质谱法（DESI-MS）。
Analyst. 2009 May;134(5):838-41. doi: 10.1039/b900069k. Epub 2009 Mar 16.
6
High Spatial Resolution Imaging Mass Spectrometry Reveals Chemical Heterogeneity Across Bacterial Microcolonies.高空间分辨率成像质谱分析揭示细菌微菌落中的化学异质性。
Anal Chem. 2019 Dec 3;91(23):14818-14823. doi: 10.1021/acs.analchem.9b03909. Epub 2019 Nov 15.
7
Profiling of acarviostatin family secondary metabolites secreted by Streptomyces coelicoflavus ZG0656 using ultraperformance liquid chromatography coupled with electrospray ionization mass spectrometry.运用超高效液相色谱联用电喷雾电离质谱法对天蓝色链霉菌ZG0656分泌的阿卡尔维他汀家族次级代谢产物进行分析。
Anal Chem. 2008 Oct 1;80(19):7554-61. doi: 10.1021/ac801117s. Epub 2008 Aug 23.
8
Imaging of lipids and metabolites using nanospray desorption electrospray ionization mass spectrometry.使用纳米喷雾解吸电喷雾电离质谱法对脂质和代谢物进行成像。
Methods Mol Biol. 2015;1203:99-106. doi: 10.1007/978-1-4939-1357-2_10.
9
Interspecies interactions stimulate diversification of the Streptomyces coelicolor secreted metabolome.种间相互作用刺激了变铅青链霉菌分泌代谢组的多样化。
mBio. 2013 Aug 20;4(4):e00459-13. doi: 10.1128/mBio.00459-13.
10
Novel method for detection of butanolides in Streptomyces coelicolor culture broth, using a His-tagged receptor (ScbR) and mass spectrometry.利用带有组氨酸标签的受体（ScbR）和质谱法检测天蓝色链霉菌培养液中丁醇内酯的新方法。
Appl Environ Microbiol. 2005 Sep;71(9):5050-5. doi: 10.1128/AEM.71.9.5050-5055.2005.
引用本文的文献
1
Bacterial biofilm sample preparation for spatial metabolomics.用于空间代谢组学的细菌生物膜样品制备
Analyst. 2025 Jul 8. doi: 10.1039/d5an00466g.
2
Microbiome in cancer metastasis: biological insights and emerging spatial omics methods.癌症转移中的微生物组：生物学见解与新兴的空间组学方法。
Front Cell Infect Microbiol. 2025 Jun 4;15:1559870. doi: 10.3389/fcimb.2025.1559870. eCollection 2025.
3
Rapid and Robust Workflows Using Different Ionization, Computation, and Visualization Approaches for Spatial Metabolome Profiling of Microbial Natural Products in .使用不同电离、计算和可视化方法对微生物天然产物进行空间代谢组分析的快速且强大的工作流程
ACS Meas Sci Au. 2024 Oct 21;4(6):668-677. doi: 10.1021/acsmeasuresciau.4c00035. eCollection 2024 Dec 18.
4
A Snapshot, Using a Multi-Omic Approach, of the Metabolic Cross-Talk and the Dynamics of the Resident Microbiota in Ripening Cheese Inoculated with .采用多组学方法对接种……的成熟奶酪中代谢相互作用及常驻微生物群动态变化的快照
Foods. 2024 Jun 18;13(12):1912. doi: 10.3390/foods13121912.
5
Metabolic insights from mass spectrometry imaging of biofilms: A perspective from model microorganisms.基于代谢组学成像的生物膜代谢研究：来自模式微生物的视角。
Methods. 2024 Apr;224:21-34. doi: 10.1016/j.ymeth.2024.01.014. Epub 2024 Jan 29.
6
MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology.基质辅助激光解吸电离质谱成像技术：现代微生物学的潜在变革者。
Cells. 2022 Dec 2;11(23):3900. doi: 10.3390/cells11233900.
7
The Exploration of Microbial Natural Products and Metabolic Interaction Guided by Mass Spectrometry Imaging.基于质谱成像的微生物天然产物及代谢相互作用探索
Bioengineering (Basel). 2022 Nov 18;9(11):707. doi: 10.3390/bioengineering9110707.
8
Lipopeptide Interplay Mediates Molecular Interactions between Soil Bacilli and Pseudomonads.脂肽相互作用介导土壤芽孢杆菌和假单胞菌之间的分子相互作用。
Microbiol Spectr. 2021 Dec 22;9(3):e0203821. doi: 10.1128/spectrum.02038-21. Epub 2021 Dec 8.
9
Antifungal potential of secondary metabolites involved in the interaction between citrus pathogens.参与柑橘病原菌相互作用的次生代谢物的抗真菌潜力。
Sci Rep. 2019 Dec 9;9(1):18647. doi: 10.1038/s41598-019-55204-9.
10
Surface-Induced Dissociation: An Effective Method for Characterization of Protein Quaternary Structure.表面诱导解离：一种用于蛋白质四级结构表征的有效方法。
Anal Chem. 2019 Jan 2;91(1):190-209. doi: 10.1021/acs.analchem.8b05071. Epub 2018 Dec 18.
本文引用的文献
1
Translating metabolic exchange with imaging mass spectrometry.利用成像质谱法对代谢物交换进行翻译。
Nat Chem Biol. 2009 Dec;5(12):885-7. doi: 10.1038/nchembio.252. Epub 2009 Nov 8.
2
Drug discovery and natural products: end of an era or an endless frontier?药物发现与天然产物：一个时代的终结还是无尽的前沿？
Science. 2009 Jul 10;325(5937):161-5. doi: 10.1126/science.1168243.
3
In vivo recognition of Bacillus subtilis by desorption electrospray ionization mass spectrometry (DESI-MS).体内鉴定枯草芽孢杆菌的解吸电喷雾电离质谱法（DESI-MS）。
Analyst. 2009 May;134(5):838-41. doi: 10.1039/b900069k. Epub 2009 Mar 16.
4
Desorption electrospray ionization mass spectrometry reveals surface-mediated antifungal chemical defense of a tropical seaweed.解吸电喷雾电离质谱法揭示了一种热带海藻的表面介导抗真菌化学防御机制。
Proc Natl Acad Sci U S A. 2009 May 5;106(18):7314-9. doi: 10.1073/pnas.0812020106. Epub 2009 Apr 6.
5
Mass spectrometric imaging of lipids using desorption electrospray ionization.使用解吸电喷雾电离技术对脂质进行质谱成像。
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Sep 15;877(26):2883-9. doi: 10.1016/j.jchromb.2008.12.058. Epub 2008 Dec 31.
6
The origins of 168, W23, and other Bacillus subtilis legacy strains.168、W23及其他枯草芽孢杆菌传统菌株的起源。
J Bacteriol. 2008 Nov;190(21):6983-95. doi: 10.1128/JB.00722-08. Epub 2008 Aug 22.
7
In situ localisation and quantification of surfactins in a Bacillus subtilis swarming community by imaging mass spectrometry.通过成像质谱法对枯草芽孢杆菌群体游动群落中的表面活性素进行原位定位和定量分析。
Proteomics. 2008 Sep;8(18):3682-91. doi: 10.1002/pmic.200701025.
8
Mining microbial genomes for new natural products and biosynthetic pathways.挖掘微生物基因组以寻找新的天然产物和生物合成途径。
Microbiology (Reading). 2008 Jun;154(Pt 6):1555-1569. doi: 10.1099/mic.0.2008/018523-0.
9
Droplet dynamics and ionization mechanisms in desorption electrospray ionization mass spectrometry.解吸电喷雾电离质谱中的液滴动力学与电离机制
Anal Chem. 2006 Dec 15;78(24):8549-55. doi: 10.1021/ac0615807.
10
Direct high-resolution peptide and protein analysis by desorption electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry.通过解吸电喷雾电离傅里叶变换离子回旋共振质谱法进行直接高分辨率肽和蛋白质分析。
Rapid Commun Mass Spectrom. 2006;20(22):3409-11. doi: 10.1002/rcm.2759.
Zotero 插件