Nishimura Kohei, Fukagawa Tatsuo, Takisawa Haruhiko, Kakimoto Tatsuo, Kanemaki Masato
Department of Biological Sciences, Osaka University, Japan.
Nat Methods. 2009 Dec;6(12):917-22. doi: 10.1038/nmeth.1401. Epub 2009 Nov 15.
Plants have evolved a unique system in which the plant hormone auxin directly induces rapid degradation of the AUX/IAA family of transcription repressors by a specific form of the SCF E3 ubiquitin ligase. Other eukaryotes lack the auxin response but share the SCF degradation pathway, allowing us to transplant the auxin-inducible degron (AID) system into nonplant cells and use a small molecule to conditionally control protein stability. The AID system allowed rapid and reversible degradation of target proteins in response to auxin and enabled us to generate efficient conditional mutants of essential proteins in yeast as well as cell lines derived from chicken, mouse, hamster, monkey and human cells, thus offering a powerful tool to control protein expression and study protein function.
植物进化出了一种独特的系统，在该系统中，植物激素生长素通过特定形式的SCF E3泛素连接酶直接诱导转录抑制因子AUX/IAA家族的快速降解。其他真核生物缺乏生长素反应，但共享SCF降解途径，这使我们能够将生长素诱导的降解子（AID）系统移植到非植物细胞中，并使用小分子有条件地控制蛋白质稳定性。AID系统能够响应生长素快速且可逆地降解靶蛋白，使我们能够在酵母以及源自鸡、小鼠、仓鼠、猴子和人类细胞的细胞系中产生必需蛋白的高效条件性突变体，从而提供了一个控制蛋白质表达和研究蛋白质功能的强大工具。
