Dix D J, Eisenberg B R
Department of Physiology and Biophysics, University of Illinois, Chicago 60680.
Dev Biol. 1991 Feb;143(2):422-6. doi: 10.1016/0012-1606(91)90093-i.
Myosin mRNA distribution among subcellular compartments of anterior tibialis muscles in rabbit is monitored by in situ hybridization. A high density of mRNA was widely distributed throughout myotubes from 29-day fetal muscle and from regenerating adult muscle. All cytoplasmic spaces contained mRNA except where scattered myofibrils and centrally located nuclei were found. In fibers from 22-week-old rabbits, myosin mRNA was concentrated under the sarcolemma and excluded from the consolidated myofibrils and peripheral nuclei. The dispersal of mRNA through the cytoplasm in myotubes suggests that translation of myosin is widespread and that rapid myofibril assembly can occur throughout the fiber.
通过原位杂交监测兔胫前肌亚细胞区室中肌球蛋白mRNA的分布。在29天胎儿肌肉和再生成年肌肉的肌管中，高密度的mRNA广泛分布。除了发现有散在肌原纤维和位于中央的细胞核的区域外，所有细胞质空间都含有mRNA。在22周龄兔子的纤维中，肌球蛋白mRNA集中在肌膜下，而在整合的肌原纤维和外周核中没有。mRNA在肌管细胞质中的分散表明肌球蛋白的翻译是广泛存在的，并且整个纤维都能快速进行肌原纤维组装。
