Hesketh J, Campbell G, Loveridge N
Division of Biochemical Sciences, Rowett Research Institute, Aberdeen, Scotland, U.K.
Biochem J. 1991 Oct 1;279 ( Pt 1)(Pt 1):309-10. doi: 10.1042/bj2790309.
Hybridization in situ with riboprobes to the myosin heavy-chain slow isoform showed that, in the rat soleus muscle, the myosin heavy-chain mRNA was distributed throughout the myofibres. There was greater density of autoradiographic grains in the subsarcolemmal regions of the fibres, but there was also a considerable number of grains in the core myofibrillar region of the fibres. Microdensitometry showed that the grain density in the myofibrillar region was approximately half that in the subsarcolemmal rim; this would correspond to some 70% of the mRNA being present in the myofibrillar region. The results are consistent with the hypothesis that myosin is synthesized on polyribosomes present in the intermyofibrillar cytoplasm.
用核糖核酸探针与肌球蛋白重链慢异构体进行原位杂交显示，在大鼠比目鱼肌中，肌球蛋白重链mRNA分布于整个肌纤维。在纤维的肌膜下区域放射自显影片颗粒密度更高，但在纤维的肌原纤维核心区域也有相当数量的颗粒。显微密度测定表明，肌原纤维区域的颗粒密度约为肌膜下边缘区域的一半；这相当于约70%的mRNA存在于肌原纤维区域。这些结果与肌球蛋白在肌原纤维间细胞质中存在的多核糖体上合成的假说一致。
