Nuclear Medicine Department, Shanghai University of Traditional Chinese Medicine affiliated PuTuo Hospital, PuTuo District, No. 164, LanXi Rd, Shanghai 200062, China.
In Vitro Cell Dev Biol Anim. 2010 Jan;46(1):60-71. doi: 10.1007/s11626-009-9240-3.
The adipogenesis effect of fibroblast growth factor 10 (FGF10) has been demonstrated in many studies. The aim of this study is to render a novel method which can continuously induce hypodermal adipose-derived stem cell (ADSC) differentiation and maturation in vivo and in vitro using FGF10. We constructed a recombinant pcDNA3.0-FGF10-MSC which can continuously express FGF10 by transfected FGF10 into a human mesenchymal stem cell (MSC) clone, and we cultured ADSCs from human subcutaneous resected adipose tissue. An in vitro and in vivo coculture system of pcDNA3.0-FGF10-MSC and ADSCs was then established. We observed the characteristics of ADSCs, monitored the adipogenesis-related transcription factor CAAT/enhancer binding protein-β, peroxisome proliferator-activated receptor-γ, and measured the adipose tissue layer of carrier animals. The results showed that FGF10 secreted from pcDNA3.0-FGF10-MSC could induce ADSC differentiation into mature adipocytes consistently. The study demonstrated that FGF10 can promote the adipogenesis effect in situ, and the autotransplantation of a carrier continuously secreting FGF10 may be utilized for increasing local subcutaneous adipose tissue in cosmetology.
成纤维细胞生长因子 10（FGF10）的脂肪生成作用已在许多研究中得到证实。本研究旨在提供一种新方法，通过将 FGF10 转染到人类间充质干细胞（MSC）克隆中，在体内和体外连续诱导真皮脂肪来源干细胞（ADSC）分化和成熟。我们构建了一种可连续表达 FGF10 的重组 pcDNA3.0-FGF10-MSC，并从人皮下切除的脂肪组织中培养 ADSC。然后建立了 pcDNA3.0-FGF10-MSC 和 ADSC 的体外和体内共培养系统。我们观察了 ADSC 的特征，监测了脂肪生成相关转录因子 CAAT/增强子结合蛋白-β、过氧化物酶体增殖物激活受体-γ，并测量了载体动物的脂肪组织层。结果表明，pcDNA3.0-FGF10-MSC 分泌的 FGF10 可持续诱导 ADSC 分化为成熟脂肪细胞。该研究表明 FGF10 可促进原位脂肪生成作用，并且持续分泌 FGF10 的载体的自体移植可用于增加美容局部皮下脂肪组织。
