高分辨率原子力显微镜观察 DNA。

High-resolution atomic force microscopy of DNA.

机构信息

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.

出版信息

Biochemistry (Mosc). 2009 Oct;74(10):1150-4. doi: 10.1134/s0006297909100113.

PMID:19916928

Abstract

A method using high resolution atomic force microscopy for imaging DNA has been elaborated. Using super-sharp probes and modified graphite as support for molecule adsorption, DNA molecule images were obtained whose resolution made possible the observation of their fine structure with repeated helical motifs. The method can be used to visualize individual spread molecules of single-stranded DNA.

摘要

已经详细阐述了一种使用高分辨率原子力显微镜成像 DNA 的方法。使用超锋利的探针和经过修饰的石墨作为分子吸附的支撑，获得了 DNA 分子的图像，其分辨率使得观察其具有重复螺旋模体的精细结构成为可能。该方法可用于可视化单链 DNA 的单个展开分子。