用原子力显微镜成像DNA结构

Imaging DNA Structure by Atomic Force Microscopy.

作者信息

Pyne Alice L B, Hoogenboom Bart W

机构信息

London Centre for Nanotechnology and Department of Physics and Astronomy, University College London, 17-19 Gordon Street, London, WC1H 0AH, UK.

出版信息

Methods Mol Biol. 2016;1431:47-60. doi: 10.1007/978-1-4939-3631-1_5.

DOI:10.1007/978-1-4939-3631-1_5

PMID:27283301

Abstract

Atomic force microscopy (AFM) is a microscopy technique that uses a sharp probe to trace a sample surface at nanometre resolution. For biological applications, one of its key advantages is its ability to visualize substructure of single molecules and molecular complexes in an aqueous environment. Here, we describe the application of AFM to determine superstructure and secondary structure of surface-bound DNA. The method is also readily applicable to probe DNA-DNA interactions and DNA-protein complexes.

摘要

原子力显微镜（AFM）是一种显微镜技术，它使用尖锐的探针以纳米分辨率追踪样品表面。对于生物应用而言，其关键优势之一在于能够在水性环境中可视化单分子和分子复合物的亚结构。在此，我们描述了原子力显微镜在确定表面结合DNA的超结构和二级结构方面的应用。该方法也很容易应用于探测DNA-DNA相互作用以及DNA-蛋白质复合物。