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苏云金芽孢杆菌的高效转化需要非甲基化的质粒DNA。

Efficient transformation of Bacillus thuringiensis requires nonmethylated plasmid DNA.

作者信息

Macaluso A, Mettus A M

机构信息

Ecogen Inc., Langhorne, Pennsylvania 19047.

出版信息

J Bacteriol. 1991 Feb;173(3):1353-6. doi: 10.1128/jb.173.3.1353-1356.1991.

DOI:10.1128/jb.173.3.1353-1356.1991
PMID:1991728
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC207264/
Abstract

The transformation efficiency of Bacillus thuringiensis depends upon the source of plasmid DNA. DNA isolated from B. thuringiensis, Bacillus megaterium, or a Dam- Dcm- Escherichia coli strain efficiently transformed several B. thuringiensis strains, B. thuringiensis strains were grouped according to which B. thuringiensis backgrounds were suitable sources of DNA for transformation of other B. thuringiensis strains, suggesting that B. thuringiensis strains differ in DNA modification and restriction. Efficient transformation allowed the demonstration of developmental regulation of cloned crystal protein genes in B. thuringiensis.

摘要

苏云金芽孢杆菌的转化效率取决于质粒DNA的来源。从苏云金芽孢杆菌、巨大芽孢杆菌或一种缺失Dam和Dcm的大肠杆菌菌株中分离得到的DNA能够高效转化多种苏云金芽孢杆菌菌株。根据哪些苏云金芽孢杆菌背景是用于转化其他苏云金芽孢杆菌菌株的合适DNA来源，对苏云金芽孢杆菌菌株进行了分组，这表明不同的苏云金芽孢杆菌菌株在DNA修饰和限制方面存在差异。高效转化使得在苏云金芽孢杆菌中对克隆的晶体蛋白基因的发育调控得以证明。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eed1/207264/ce6eee758ef6/jbacter00093-0429-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eed1/207264/ed64d20471d7/jbacter00093-0429-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eed1/207264/ce6eee758ef6/jbacter00093-0429-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eed1/207264/ed64d20471d7/jbacter00093-0429-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eed1/207264/ce6eee758ef6/jbacter00093-0429-b.jpg

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