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用肽、β2微球蛋白和MHC重链工程改造的稳定单链三聚体的制备。

Preparation of stable single-chain trimers engineered with peptide, beta2 microglobulin, and MHC heavy chain.

作者信息

Hansen Ted, Yu Y Y Lawrence, Fremont Daved H

机构信息

Washington University School of Medicine, St. Louis, Missouri.

出版信息

Curr Protoc Immunol. 2009 Nov;Chapter 17:17.5.1-17.5.17. doi: 10.1002/0471142735.im1705s87.

DOI:10.1002/0471142735.im1705s87
PMID:19918946
Abstract

This unit describes a method for constructing a class I MHC molecule with a bound peptide as a single polypeptide chain, termed SCT, for single chain trimer. The component organization of the SCT appears to be widely applicable to different mouse or human MHC class I isotypes bound by different antigenic peptides. The enhanced peptide occupancy afforded by the SCT format makes these molecules effective reagents as DNA vaccines, multimeric staining reagents to enumerate CD8 T cells, and probes of lymphocyte biology.

摘要

本单元描述了一种构建具有结合肽的I类MHC分子的方法，该分子为单条多肽链，称为SCT（单链三聚体）。SCT的组成结构似乎广泛适用于与不同抗原肽结合的不同小鼠或人类I类MHC同种型。SCT形式提供的更高肽占有率使这些分子成为有效的试剂，可作为DNA疫苗、用于计数CD8 T细胞的多聚体染色试剂以及淋巴细胞生物学的探针。

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