School of Nutrition, Chung Shan Medical University, 110, Sec. 1, Chien-kuo N. Road, Taichung 40203, Taiwan.
J Agric Food Chem. 2009 Nov 25;57(22):11008-13. doi: 10.1021/jf902725s.
Fungal immunomodulatory protein, FIP-fve, has been isolated from Flammulina velutipes, and its immunomodulatory effects are believed to be associated with the enhanced activation of IFN-gamma-releasing Th1 cells. However, the mechanisms of FIP-fve-mediated signal transduction in the regulation of interferon-gamma (IFN-gamma) gene expression in human peripheral blood mononuclear cells (PBMCs) are still poorly understood. Using fluo-3 AM, we found that FIP-fve induces a rapid elevation in calcium concentration. ELISA, RT-PCR and Western blot assays demonstrated significant increases in the production and mRNA expression of IFN-gamma and protein kinase C-alpha (PKC-alpha) activation in activated PBMCs, which were abolished by EGTA, nifedipine and GO6976. In conclusion, Ca2+ release and PKC-alpha activation are required for IFN-gamma production induced by FIP-fve in PBMCs.
真菌免疫调节蛋白 FIP-fve 已从金针菇中分离出来，其免疫调节作用被认为与增强 IFN-γ释放的 Th1 细胞的激活有关。然而，FIP-fve 介导的信号转导在调节人外周血单个核细胞 (PBMC) 中干扰素-γ (IFN-γ) 基因表达的机制仍知之甚少。使用 fluo-3 AM，我们发现 FIP-fve 诱导钙浓度的快速升高。ELISA、RT-PCR 和 Western blot 分析表明，在活化的 PBMC 中 IFN-γ和蛋白激酶 C-α (PKC-α) 的产生和 mRNA 表达显著增加，而 EGTA、硝苯地平和 GO6976 则使其消失。总之，FIP-fve 在 PBMC 中诱导 IFN-γ产生需要 Ca2+释放和 PKC-α 激活。
