Marzec Lukasz, Zdrojewski Zbigniew, Liberek Tomasz, Bryl Ewa, Chmielewski Michal, Witkowski Jacek M, Rutkowski Boleslaw
Departments of Nephrology, Transplantology and Internal Medicine, Medical University of Gdansk, Gdansk, Poland.
Scand J Urol Nephrol. 2009;43(5):400-8. doi: 10.3109/00365590903089489.
Expression of heat shock protein (Hsp) 72 is one of the major mechanisms acting against cellular injury. It displays a plethora of functions, including a considerable impact on inflammation. The aim of this study was to investigate Hsp72 expression in blood monocytes of patients with chronic kidney disease (CKD).
Hsp72 protein level was assessed by flow cytometry in blood monocytes of predialysis, haemodialysis (HD) and continuous peritoneal dialysis patients, and controls. It was followed by evaluation of Hsp72 gene expression in the same cohorts by reverse transcription-polymerase chain reaction.
The level of Hsp72 protein was significantly lower in the predialysis (359+/-83 AU) and HD groups (293+/-62 AU) than in controls (405+/-51 AU) (p<0.01 and p<0.001, respectively). The amount of mRNA was significantly lower only in the HD group, compared with controls (0.39+/-0.10 vs 0.48+/-0.10, p<0.01). In the predialysis group, there were negative correlations between Hsp72 protein level and serum creatinine concentration, blood urea nitrogen and C-reactive protein.
This study demonstrates that uraemic toxicity decreases expression of Hsp72. Attenuation of Hsp 72 expression in uraemia, found in the present study, could contribute to the inflammatory state, a common complication in CKD patients.
热休克蛋白（Hsp）72的表达是抵抗细胞损伤的主要机制之一。它具有多种功能，包括对炎症有相当大的影响。本研究的目的是调查慢性肾脏病（CKD）患者血液单核细胞中Hsp72的表达情况。
采用流式细胞术评估未透析、血液透析（HD）和持续性腹膜透析患者以及对照组血液单核细胞中Hsp72蛋白水平。随后通过逆转录聚合酶链反应评估同一队列中Hsp72基因的表达。
未透析组（359±83 AU）和HD组（293±62 AU）的Hsp72蛋白水平显著低于对照组（405±51 AU）（分别为p<0.01和p<0.001）。与对照组相比，仅HD组的mRNA量显著降低（0.39±0.10对0.48±0.10，p<0.01）。在未透析组中，Hsp72蛋白水平与血清肌酐浓度、血尿素氮和C反应蛋白之间呈负相关。
本研究表明尿毒症毒性会降低Hsp72的表达。本研究中发现的尿毒症患者Hsp72表达减弱可能导致炎症状态，这是CKD患者常见的并发症。
