Dou Bingde, Hou Beiwei, Xu Haiming, Lou Xiangyang, Chi Xiaofei, Yang Jinbin, Wang Fang, Ni Zhongfu, Sun Qixin
Institute of Plant Biotechnology, Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huai'an, People's Republic of China.
Genet Res (Camb). 2009 Oct;91(5):337-43. doi: 10.1017/S0016672309990218.
Studies on inheritance of fertility are of great importance in wheat breeding. Although substantial progress has been achieved in molecular characterization of male sterility and fertility restoration recently, little effort has been devoted to female sterility. To identify the gene(s) controlling female sterility in wheat efficiently, an investigation was conducted for the seed setting ratio using a set of F2 populations derived from the cross between a female sterile line XND126 and an elite cultivar Gaocheng 8901. Bulked segregation analysis (BSA) method and recessive class approach were adopted to screen for SSR markers potentially linked to female fertility gene loci in 2005. Out of 1080 SSRs in wheat genome, eight markers on chromosome 2D showed a clear difference between two disparate bulks and small recombination frequency values, suggesting a strong linkage signal to the sterility gene. Based on the candidate linked markers, partial linkage maps were constructed with Mapmaker 3.0 (EXP) instead of whole genome maps, and quantitative trait locus (QTL) mapping was implemented with software QTLNetwork 2.0. A major gene locus designated as taf1, was located on chromosome 2DS. The above result was confirmed by the analysis for 2007 data, and taf1 was identified on the same chromosome 2DS with a confidence interval of 2.4 cM, which could explain 44.99% of phenotypic variation. These results provided fundamental information for fine mapping studies and laid the groundwork for wheat fertility genetic studies.
育性遗传研究在小麦育种中具有重要意义。尽管近年来在雄性不育和育性恢复的分子特征研究方面取得了显著进展，但对雌性不育的研究投入较少。为了高效鉴定控制小麦雌性不育的基因，利用雌性不育系XND126与优良品种藁城8901杂交衍生的一套F2群体，对结实率进行了调查。2005年采用混合分离分析法（BSA）和隐性类群法筛选与雌性育性基因位点可能连锁的SSR标记。在小麦基因组的1080个SSR中，2D染色体上的8个标记在两个不同的混合群体间表现出明显差异，且重组频率值较小，表明与不育基因有较强的连锁信号。基于候选连锁标记，使用Mapmaker 3.0（EXP）构建了部分连锁图谱而非全基因组图谱，并使用软件QTLNetwork 2.0进行了数量性状位点（QTL）定位。一个命名为taf1的主基因位点位于2DS染色体上。对2007年数据的分析证实了上述结果，taf1在同一条2DS染色体上被鉴定出来，置信区间为2.4 cM，可解释44.99%的表型变异。这些结果为精细定位研究提供了基础信息，为小麦育性遗传研究奠定了基础。
