Durmaz A, Kurt B, Ongoru O, Karahatay S, Gerek M, Yalcin S
Department of Otorhinolaryngology Head and Neck Surgery, Gulhane Military Medical Academy, Ankara, Turkey.
J Laryngol Otol. 2010 Feb;124(2):194-8. doi: 10.1017/S0022215109991630. Epub 2009 Nov 19.
Fascin is an actin-binding protein which is expressed in the basal areas of healthy squamous epithelium. Although overexpression of fascin has been shown in many tumours, the relationship between fascin and laryngeal squamous cell carcinoma has not previously been investigated, to the best of our knowledge. This study aimed to investigate the relationship between fascin expression and tumour behaviour in 30 cases of laryngeal squamous cell carcinoma.
For all lesions, a section of paraffin-embedded tissue was immunohistochemically stained for fascin. The percentage of positive, stained cells was scored from one to five (one = 0-5 per cent, two = 6-25 per cent, three = 26-50 per cent, four = 51-75 per cent and five = 76-100 per cent), and the staining intensity from one to three (one = mild, two = moderate and three = strong). A total immunohistochemical fascin expression score was obtained by multiplying the staining percentage and intensity. The relationship between the total fascin score and each case's age, sex, tumour localisation, tumour-node-metastasis stage and differentiation was evaluated statistically.
Various amounts of fascin expression were observed in all cases. There was a statistically significant relationship between high levels of fascin expression (i.e. a total fascin score of 10 or more) and the cases' tumour stage (p = 0.022), node stage (p = 0.024) and clinical stage (p = 0.014). In addition, worsening tumour differentiation was associated with an increasing fascin score, but this finding was statistically insignificant.
These results suggest that laryngeal squamous cell carcinomas with high levels of fascin expression may be more aggressive than those with low expression levels. Further studies with larger series are needed to support these results and to clarify rationales.
Fascin是一种肌动蛋白结合蛋白，在健康鳞状上皮的基底区域表达。尽管已证实在许多肿瘤中Fascin表达上调，但据我们所知，此前尚未研究过Fascin与喉鳞状细胞癌之间的关系。本研究旨在探讨30例喉鳞状细胞癌中Fascin表达与肿瘤行为之间的关系。
对所有病变，取一段石蜡包埋组织进行Fascin免疫组织化学染色。阳性染色细胞的百分比评分为1至5分（1分=0-5%，2分=6-25%，3分=26-50%，4分=51-75%，5分=76-100%），染色强度评分为1至3分（1分=轻度，2分=中度，3分=重度）。通过将染色百分比与强度相乘获得总的免疫组织化学Fascin表达评分。对总的Fascin评分与每个病例的年龄、性别、肿瘤定位、肿瘤-淋巴结-转移分期及分化程度之间的关系进行统计学评估。
所有病例均观察到不同程度的Fascin表达。Fascin高表达（即总的Fascin评分≥10）与病例的肿瘤分期（p=0.022）、淋巴结分期（p=0.024）及临床分期（p=0.014）之间存在统计学显著相关性。此外，肿瘤分化程度越差，Fascin评分越高，但这一发现无统计学意义。
这些结果表明，Fascin高表达的喉鳞状细胞癌可能比低表达者更具侵袭性。需要进一步开展更大样本量的研究来支持这些结果并阐明其原理。
