Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
J Virol. 2010 Feb;84(3):1631-6. doi: 10.1128/JVI.01482-09. Epub 2009 Nov 18.
Induction of antibodies that neutralize a broad range of human immunodeficiency virus type 1 (HIV-1) isolates is a major goal of vaccine development. To study natural examples of broad neutralization, we analyzed sera from 103 HIV-1-infected subjects. Among progressor patients, 20% of sera neutralized more than 75% of a panel of 20 diverse viral isolates. Little activity was observed in sera from long-term nonprogressors (elite controllers). Breadth of neutralization was correlated with viral load, but not with CD4 count, history of past antiretroviral use, age, gender, race/ethnicity, or route of exposure. Clustering analysis of sera by a novel method identified a statistically robust subgrouping of sera that demonstrated broad and potent neutralization activity.
诱导能够中和广泛的人类免疫缺陷病毒 1 型（HIV-1）分离株的抗体是疫苗开发的主要目标。为了研究广泛中和的自然实例，我们分析了 103 名 HIV-1 感染个体的血清。在进展者患者中，20%的血清能够中和 20 种不同病毒分离株的 75%以上。在长期非进展者（精英控制者）的血清中几乎没有观察到活性。中和的广度与病毒载量相关，但与 CD4 计数、过去使用抗逆转录病毒药物的历史、年龄、性别、种族/民族或暴露途径无关。通过一种新方法对血清进行聚类分析，确定了一个具有统计学意义的血清亚群，这些血清表现出广泛而有效的中和活性。
