Pernas Maria, Sanchez-Merino Victor, Casado Concepcion, Merino-Mansilla Alberto, Olivares Isabel, Yuste Eloisa, Lopez-Galindez Cecilio
Centro Nacional de Microbiología (CNM), Instituto de Salud Carlos III, Majadahonda, Madrid 28220, Spain.
AIDS Research Unit, Institut d´Investigacions Biomediquès August Pi i Sunyer, Barcelona, Spain; HIVACAT, Barcelona, Spain.
PLoS One. 2015 Aug 10;10(8):e0134054. doi: 10.1371/journal.pone.0134054. eCollection 2015.
This study evaluated the neutralization breadth in dually infected (DI) HIV-1 long-term non-progressor elite controller patients (LTNP-EC) using a representative minipanel of 6 viruses from 5 different subtypes. Our results showed an improved neutralization breadth in DI LTNP-EC patients when compared with matched LTNP single-infected patients. The role of viral diversity in neutralization was estimated with the Shannon Entropy and the p-distance in viral quasispecies. We found a positive correlation between neutralization breadth and diversity within the viral quasispecies. This correlation could explain why a group of LTNP-EC patients developed a broad neutralizing response despite having undetectable levels of viremia.
本研究使用来自5种不同亚型的6种病毒的代表性小型病毒组，评估了双重感染（DI）的HIV-1长期非进展者精英控制者患者（LTNP-EC）的中和广度。我们的结果显示，与匹配的单一感染LTNP患者相比，DI LTNP-EC患者的中和广度有所提高。利用香农熵和病毒准种中的p距离估计了病毒多样性在中和中的作用。我们发现中和广度与病毒准种内的多样性之间存在正相关。这种相关性可以解释为什么一组LTNP-EC患者尽管病毒血症水平检测不到，但仍产生了广泛的中和反应。
