鞘氨醇单胞菌属SYK-6中没食子酸双加氧酶DesB的结晶及初步晶体学分析
Crystallization and preliminary crystallographic analysis of gallate dioxygenase DesB from Sphingobium sp. SYK-6.
作者信息
Sugimoto Keisuke, Yamamoto Yoshihiro, Antoni Siswanto, Senda Miki, Kasai Daisuke, Masai Eiji, Fukuda Masao, Senda Toshiya
机构信息
Department of Materials Chemistry, Asahikawa National College of Technology, Shunko-dai, Asahikawa, Hokkaido, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Nov 1;65(Pt 11):1171-4. doi: 10.1107/S1744309109041086. Epub 2009 Oct 30.
Abstract
Gallate dioxygenase (DesB) from Sphingobium sp. SYK-6, which belongs to the type II extradiol dioxygenase family, was purified and crystallized using the hanging-drop vapour-diffusion method. Two crystal forms were obtained. The form I crystal belonged to space group C2, with unit-cell parameters a = 136.2, b = 53.6, c = 55.1 angstrom, beta = 112.8 degrees, and diffracted to 1.6 angstrom resolution. The form II crystal belonged to space group P2(1), with unit-cell parameters a = 56.2, b = 64.7, c = 116.1 angstrom, beta = 95.1 degrees, and diffracted to 1.9 angstrom resolution. A molecular-replacement calculation using LigAB as a search model yielded a satisfactory solution for both crystal forms.
摘要
来自鞘氨醇单胞菌属SYK-6的没食子酸双加氧酶（DesB）属于II型间苯二酚双加氧酶家族，采用悬滴气相扩散法进行纯化和结晶。获得了两种晶体形式。I型晶体属于空间群C2，晶胞参数a = 136.2、b = 53.6、c = 55.1埃，β = 112.8°，衍射分辨率为1.6埃。II型晶体属于空间群P2(1)，晶胞参数a = 56.2、b = 64.7、c = 116.1埃，β = 95.1°，衍射分辨率为1.9埃。以LigAB为搜索模型进行分子置换计算，两种晶体形式均得到了满意的结果。
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