Umeda Takashi, Katsuki Junichi, Ashikawa Yuji, Usami Yusuke, Inoue Kengo, Noguchi Haruko, Fujimoto Zui, Yamane Hisakazu, Nojiri Hideaki
Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Nov 1;66(Pt 11):1480-3. doi: 10.1107/S1744309110034949. Epub 2010 Oct 28.
Carbazole 1,9a-dioxygenase (CARDO) is the initial dioxygenase in the carbazole-degradation pathway of Novosphingobium sp. KA1. The CARDO from KA1 consists of a terminal oxygenase (Oxy), a putidaredoxin-type ferredoxin and a ferredoxin reductase. The Oxy from Novosphingobium sp. KA1 was crystallized at 277 K using the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant. Diffraction data were collected to a resolution of 2.1 Å. The crystals belonged to the monoclinic space group P2(1). Self-rotation function analysis suggested that the asymmetric unit contained two Oxy trimers; the Matthews coefficient and solvent content were calculated to be 5.9 Å(3) Da(-1) and 79.1%, respectively.
咔唑1,9a-双加氧酶（CARDO）是新鞘氨醇菌KA1咔唑降解途径中的初始双加氧酶。来自KA1的CARDO由一个末端加氧酶（Oxy）、一个恶臭假单胞菌型铁氧还蛋白和一个铁氧还蛋白还原酶组成。使用硫酸铵作为沉淀剂，通过悬滴气相扩散法在277 K下使新鞘氨醇菌KA1的Oxy结晶。收集了分辨率为2.1 Å的衍射数据。晶体属于单斜空间群P2(1)。自旋转函数分析表明，不对称单元包含两个Oxy三聚体；计算得出的马修斯系数和溶剂含量分别为5.9 Å(3) Da(-1)和79.1%。
