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本文引用的文献

1
Characterization of a serine/threonine kinase involved in virulence of Staphylococcus aureus.一种参与金黄色葡萄球菌毒力的丝氨酸/苏氨酸激酶的特性分析。
J Bacteriol. 2009 Jul;191(13):4070-81. doi: 10.1128/JB.01813-08. Epub 2009 Apr 24.
2
Modulation of cell wall structure and antimicrobial susceptibility by a Staphylococcus aureus eukaryote-like serine/threonine kinase and phosphatase.金黄色葡萄球菌类真核丝氨酸/苏氨酸激酶和磷酸酶对细胞壁结构及抗菌敏感性的调节作用
Infect Immun. 2009 Apr;77(4):1406-16. doi: 10.1128/IAI.01499-08. Epub 2009 Feb 2.
3
A eukaryotic-like Ser/Thr kinase signals bacteria to exit dormancy in response to peptidoglycan fragments.一种类似真核生物的丝氨酸/苏氨酸激酶可使细菌响应肽聚糖片段而退出休眠状态。
Cell. 2008 Oct 31;135(3):486-96. doi: 10.1016/j.cell.2008.08.039.
4
The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis.青霉素结合蛋白：结构及其在肽聚糖生物合成中的作用
FEMS Microbiol Rev. 2008 Mar;32(2):234-58. doi: 10.1111/j.1574-6976.2008.00105.x. Epub 2008 Feb 11.
5
Evolution of transmembrane protein kinases implicated in coordinating remodeling of gram-positive peptidoglycan: inside versus outside.参与协调革兰氏阳性菌肽聚糖重塑的跨膜蛋白激酶的演变：内膜与外膜的比较
J Bacteriol. 2006 Nov;188(21):7470-6. doi: 10.1128/JB.00800-06. Epub 2006 Aug 25.
6
Role of protein phosphorylation on serine/threonine and tyrosine in the virulence of bacterial pathogens.丝氨酸/苏氨酸和酪氨酸蛋白磷酸化在细菌病原体毒力中的作用。
J Mol Microbiol Biotechnol. 2005;9(3-4):198-213. doi: 10.1159/000089648.
7
New approaches to high-throughput phasing.高通量定相的新方法。
Curr Opin Struct Biol. 2002 Oct;12(5):674-8. doi: 10.1016/s0959-440x(02)00372-x.
8
The PASTA domain: a beta-lactam-binding domain.
Trends Biochem Sci. 2002 Sep;27(9):438. doi: 10.1016/s0968-0004(02)02164-3.
9
Implementation of molecular replacement in AMoRe.在AMoRe中分子置换的实施。
Acta Crystallogr D Biol Crystallogr. 2001 Oct;57(Pt 10):1367-72. doi: 10.1107/s0907444901012422. Epub 2001 Sep 21.
10
Crystal structure of PBP2x from a highly penicillin-resistant Streptococcus pneumoniae clinical isolate: a mosaic framework containing 83 mutations.来自一株对青霉素高度耐药的肺炎链球菌临床分离株的PBP2x晶体结构：一个包含83个突变的嵌合框架
J Biol Chem. 2001 Nov 30;276(48):45106-12. doi: 10.1074/jbc.M107608200. Epub 2001 Sep 11.

来自人类病原体金黄色葡萄球菌的丝氨酸/苏氨酸激酶Stk1的三个PASTA结构域的结晶及初步X射线衍射研究。

Crystallization and initial X-ray diffraction study of the three PASTA domains of the Ser/Thr kinase Stk1 from the human pathogen Staphylococcus aureus.

作者信息

Paracuellos Patricia, Ballandras Allison, Robert Xavier, Cozzone Alain J, Duclos Bertrand, Gouet Patrice

机构信息

Institut de Biologie et Chimie des Protéines, UMR CNRS Université de Lyon, France.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Nov 1;65(Pt 11):1187-9. doi: 10.1107/S174430910904250X. Epub 2009 Oct 30.

DOI:10.1107/S174430910904250X
PMID:19923747
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2777055/
Abstract

PASTA subunits (approximately 70 amino acids) are specific to bacterial serine/threonine kinases and to penicillin-binding proteins (PBPs) and are involved in the synthesis of peptidoglycan. The human pathogen Staphylococcus aureus contains a serine/threonine kinase, Stk1, which plays a major role in virulence. A recombinant His-tagged portion of the extracellular domain of Stk1 containing three PASTA subunits has been crystallized using zinc sulfate as a crystallizing agent. The crystals belonged to the tetragonal space group P4(1)22, with unit-cell parameters a = 68.0, b = 68.0, c = 158.1 angstrom. Structure determination by the MAD method is now in progress.

摘要

PASTA亚基（约70个氨基酸）是细菌丝氨酸/苏氨酸激酶和青霉素结合蛋白（PBPs）所特有的，参与肽聚糖的合成。人类病原体金黄色葡萄球菌含有一种丝氨酸/苏氨酸激酶Stk1，它在毒力方面起主要作用。使用硫酸锌作为结晶剂，已使含有三个PASTA亚基的Stk1细胞外结构域的重组His标签部分结晶。晶体属于四方晶系空间群P4(1)22，晶胞参数a = 68.0，b = 68.0，c = 158.1埃。目前正在通过MAD方法进行结构测定。