Research Center for Food and Development, CIAD, A.C., Carretera Gustavo Enrique Astiazaran Rosas, No. 46, Col. La Victoria, Hermosillo 83304, Sonora, Mexico.
IATE, INRAE, Institut Agro, University Montpellier, CEDEX 01, 34060 Montpellier, France.
Molecules. 2021 Apr 23;26(9):2478. doi: 10.3390/molecules26092478.
Ferulated polysaccharides such as pectin and arabinoxylan form covalent gels which are attractive for drug delivery or cell immobilization. is a probiotic yeast known for providing humans with health benefits; however, its application is limited by viability loss under environmental stress. In this study, ferulated pectin from sugar beet solid waste (SBWP) and ferulated arabinoxylan from maize bioethanol waste (AX) were used to form a covalent mixed gel, which was in turn used to entrap (2.08 × 10 cells/mL) in microbeads using electrospray. SBWP presented a low degree of esterification (30%), which allowed gelation through Ca, making it possible to reduce microbead aggregation and coalescence by curing the particles in a 2% CaCl cross-linking solution. SBWP/AX and SBWP/AX+ microbeads presented a diameter of 214 and 344 µm, respectively, and a covalent cross-linking content (dimers di-FA and trimer tri-FA of ferulic acid) of 1.15 mg/g polysaccharide. The 8-5', 8--4'and 5-5'di-FA isomers proportions were 79%, 18%, and 3%, respectively. Confocal laser scanning microscopy images of propidium iodide-stained yeasts confirmed cell viability before and after microbeads preparation by electrospray. SBWP/AX capability to entrap would represent an alternative for probiotic immobilization in tailored biomaterials and an opportunity for sustainable waste upcycling to value-added products.
阿魏酸酯化多糖（如果胶和阿拉伯木聚糖）形成共价凝胶，对药物输送或细胞固定化具有吸引力。酵母 是一种益生菌，以提供人类健康益处而闻名；然而，其应用受到环境胁迫下活力丧失的限制。在本研究中，使用甜菜固体废弃物中的阿魏酸酯化果胶（SBWP）和玉米生物乙醇废弃物中的阿魏酸酯化阿拉伯木聚糖（AX）形成共价混合凝胶，进而使用电喷雾将 （2.08×10 个细胞/mL）包埋在微珠中。SBWP 的酯化度低（30%），允许通过 Ca 进行凝胶化，通过在 2% CaCl 交联溶液中固化颗粒，使微珠聚集和聚结减少成为可能。SBWP/AX 和 SBWP/AX+微珠的直径分别为 214 和 344 µm，共价交联含量（阿魏酸的二聚体 di-FA 和三聚体 tri-FA）为 1.15 mg/g 多糖。8-5'、8--4'和 5-5' di-FA 异构体的比例分别为 79%、18%和 3%。碘化丙啶染色酵母的共焦激光扫描显微镜图像证实了电喷雾制备微珠前后细胞的活力。SBWP/AX 包埋 的能力将为定制生物材料中益生菌固定化提供替代方案，并为可持续废物升级为增值产品提供机会。
