Department of Chemistry and Biochemistry, Denison University, Granville, USA.
Curr Microbiol. 2010 Apr;60(4):274-9. doi: 10.1007/s00284-009-9537-1. Epub 2009 Nov 19.
Putative penicillin-binding proteins (PBPs) were identified in the genome of the Burkholderia cenocepacia strain J2315 based on homology to E. coli PBPs. The three sequences identified as homologs of E. coli PBP1a, BCAL2021, BCAL0274, and BCAM2632, were cloned and expressed as His(6)-tagged fusion proteins in E. coli. The fusion proteins were isolated and shown to bind beta-lactams, indicating these putative PBPs have penicillin-binding activity.
根据与大肠杆菌 PBPs 的同源性，在伯克霍尔德氏菌中鉴定出假定的青霉素结合蛋白（PBPs）。从基因组中鉴定出与大肠杆菌 PBP1a、BCAL2021、BCAL0274 和 BCAM2632 同源的 3 个序列，将这些序列克隆并在大肠杆菌中表达为 His(6)-标记的融合蛋白。分离出融合蛋白并证明其结合β-内酰胺类抗生素，表明这些假定的 PBPs 具有青霉素结合活性。
