Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Mycopathologia. 2010 Apr;169(4):287-95. doi: 10.1007/s11046-009-9251-3. Epub 2009 Nov 19.
Candidiasis is a term describing infections by yeasts from the genus Candida, and the type of infection encompassed by candidiasis ranges from superficial to systemic. Treatment of such infections often requires antifungals such as the azoles, but increased use of these drugs has led to selection of yeasts with increased resistance to these drugs. In this study, we used allicin, an allyl sulfur derivative of garlic, to demonstrate both its intrinsic antifungal activity and its synergy with the azoles, in the treatment of these yeasts in vitro. In this study, the MIC(50) and MIC(90) of allicin alone against six Candida spp. ranged from 0.05 to 25 microg/ml. However, when allicin was used in combination with fluconazole or ketoconazole, the MICs were decreased in some isolates. Our results demonstrated the existing synergistic effect between allicin and azoles in some of the Candida spp. such as C. albicans, C. glabrata and C. tropicalis, but synergy was not demonstrated in the majority of Candida spp. tested. Nonetheless, In vivo testing needs to be performed to support these findings.
念珠菌病是指由念珠菌属的酵母菌引起的感染，念珠菌病所涵盖的感染类型从浅表到全身。此类感染的治疗通常需要使用抗真菌药物，如唑类药物，但这些药物的广泛应用导致了对这些药物具有更高耐药性的酵母菌的选择。在这项研究中，我们使用大蒜中的一种烯丙基硫代衍生物大蒜素，来证明其内在的抗真菌活性及其与唑类药物的协同作用，从而在体外治疗这些酵母菌。在这项研究中，大蒜素单独对六种念珠菌属的 MIC（50）和 MIC（90）范围为 0.05 至 25μg/ml。然而，当大蒜素与氟康唑或酮康唑联合使用时，一些分离株的 MIC 值降低。我们的结果表明，大蒜素和唑类药物在一些念珠菌属（如白色念珠菌、光滑念珠菌和热带念珠菌）中存在协同作用，但在大多数测试的念珠菌属中并未显示出协同作用。尽管如此，仍需要进行体内试验来支持这些发现。
