Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran.
Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Int J Biol Macromol. 2018 Jan;106:266-276. doi: 10.1016/j.ijbiomac.2017.08.020. Epub 2017 Aug 9.
Here, using (poly(N-isopropylacrylamide)-co-oleic acid)-g-chitosan ((PNIPAAm-co-OA)-g-CS), CS copolymer-gold hybrid nanoparticles (CGH NPs) were synthesized by autoreduction of auric cations (HAuCl) in aqueous solution in the absence of any other reducing agent. The engineered thermo-sensitive CS copolymer with free amino groups could reduce auric cations and stabilized the resultant NPs. CGH NPs were prepared using different concentrations of CS copolymer (0.1-1% w/v) and HAuCl (50-500μL, 0.2% w/v). They were characterized in terms of structure, surface Plasmon band, zeta potential, atomic absorption, stability, size and size distribution. The obtained CGH NPs showed a size range of 80-100nm and high stability at different pHs with no observable agglomeration/sedimentation for couple of months. The loading efficiency of erlotinib (ETB) in the CGH NPs was about 30%. The ETB was released from the CGH NPs in a thermo-responsive manner. FACS flow cytometry analysis confirmed high cellular uptake (85.81%) of CGH NPs by A549 cells. The cytotoxicity evaluations proved the cytocompatibility and high anti-tumor effect of the engineered CGH NPs. Based on these findings, having used thermo-sensitive CS copolymer, CGH NPs were obtained in one-pot procedure, which could be considered as stimuli-responsive delivery system with potential biomedical applications.
在这里，使用（聚（N-异丙基丙烯酰胺）-co-油酸）-g-壳聚糖（（PNIPAAm-co-OA）-g-CS），通过在没有任何其他还原剂的情况下在水溶液中自还原金阳离子（HAuCl）合成了壳聚糖共聚物-g-金杂化纳米粒子（CGH NPs）。具有游离氨基的工程热敏性 CS 共聚物可以还原金阳离子并稳定所得 NPs。CGH NPs 是使用不同浓度的 CS 共聚物（0.1-1%w/v）和 HAuCl（50-500μL，0.2%w/v）制备的。它们的结构、表面等离子体带、zeta 电位、原子吸收、稳定性、尺寸和尺寸分布进行了表征。所得 CGH NPs 的粒径范围为 80-100nm，在不同 pH 值下具有高稳定性，在几个月内没有观察到聚集/沉淀。CGH NPs 中厄洛替尼（ETB）的载药效率约为 30%。CGH NPs 以热响应方式释放 ETB。FACS 流式细胞术分析证实 A549 细胞对 CGH NPs 的摄取率高达 85.81%。细胞毒性评价证明了工程 CGH NPs 的细胞相容性和高抗肿瘤作用。基于这些发现，使用热敏性 CS 共聚物，一锅法得到 CGH NPs，可作为具有潜在生物医学应用的刺激响应性递药系统。
