Department of Biological Sciences and Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology, Daejeon 305-701, South Korea.
Macromol Biosci. 2010 Mar 10;10(3):239-45. doi: 10.1002/mabi.200900291.
Pluronic/polyethylenimine shell crosslinked nanocapsules with embedded magnetite nanocrystals (PPMCs) were developed for magnetically triggered delivery of siRNA. The positively charged PPMCs formed stable nanosized polyelectrolyte complexes via electrostatic interactions with negatively charged siRNA-polyethylene glycol conjugate (siRNA-s-s-PEG) that was linked via a cleavable disulfide linkage. PPMC/siRNA-s-s-PEG polyelectrolyte complexes were efficiently taken up by cancer cells upon exposure to a magnet, thereby enhancing intracellular uptake and silencing effect of siRNA. The present study suggests that these novel nanomaterials could be potentially utilized for magnetically triggered delivery of various nucleic acid-based therapeutic agents.
研制了具有嵌入式磁铁矿纳米晶体的 Pluronic/聚亚乙基亚胺壳交联纳米胶囊（PPMCs），用于通过磁触发递送来输送 siRNA。带正电荷的 PPMC 与通过可裂解的二硫键连接的带负电荷的 siRNA-聚乙二醇缀合物（siRNA-s-s-PEG）通过静电相互作用形成稳定的纳米级聚电解质复合物。在磁铁的作用下，PPMC/siRNA-s-s-PEG 聚电解质复合物被癌细胞有效摄取，从而增强了 siRNA 的细胞内摄取和沉默效果。本研究表明，这些新型纳米材料可能可用于通过磁触发递送来输送各种基于核酸的治疗剂。
