Department of Clinical Sciences, College of Veterinary Medicine, Oregon State University, 227 Magruder Hall, Corvallis, OR 97333, USA.
J Vet Intern Med. 2010 Jan-Feb;24(1):97-103. doi: 10.1111/j.1939-1676.2009.0418.x.
Diarrhea is highly prevalent in racing sled dogs, although the underlying causes are poorly understood.
Clostridium perfringens enterotoxin (CPE) and Clostridium difficile Toxin A and B are associated with diarrhea in racing sled dogs.
One hundred and thirty-five sled dogs.
Freshly voided feces were obtained from 55 dogs before racing and from 80 dogs after 400 miles of racing. Samples were visually scored for diarrhea, mucus, blood, and melena. CPE and C. difficile Toxin A and B were detected by ELISA. Samples were cultured for C. perfringens, C. difficile, Campylobacter, Salmonella, and Escherichia coli O157; Giardia and Cryptosporidium spp. were detected via immunofluorescence.
Diarrhea occurred in 36% of dogs during racing, and hematochezia, fecal mucus or melena, or all 3 occurred in 57.5% of dogs. Salmonella was isolated from 78.2% of dogs before racing, and from 71.3% of dogs during racing. C. perfringens and C. difficile were isolated from 100 and 58.2% of dogs before racing, and from 95 and 36.3% of dogs during racing. Dogs were more likely to test positive for CPE during than before racing (18.8 versus 5.5%, P = .021); however, no enteropathogens or their respective toxins were significantly associated with hematochezia or diarrhea.
Sled dogs participating in long distance racing have a high prevalence of diarrhea and hematochezia that is not associated with common enteropathogens. It is possible that diarrhea and hematochezia represent the effect of prolonged exercise on the gastrointestinal tract.
腹泻在竞速雪橇犬中非常普遍，但其根本原因尚不清楚。
产气荚膜梭菌肠毒素（CPE）和艰难梭菌毒素 A 和 B 与竞速雪橇犬腹泻有关。
135 只雪橇犬。
在比赛前，从 55 只狗身上获得新鲜的粪便，在 80 只狗完成 400 英里的比赛后获得粪便。对粪便进行视觉评分，以评估腹泻、黏液、血液和柏油样便。通过 ELISA 检测 CPE 和艰难梭菌毒素 A 和 B。对粪便进行培养，以检测产气荚膜梭菌、艰难梭菌、弯曲杆菌、沙门氏菌和大肠杆菌 O157；通过免疫荧光法检测贾第虫和隐孢子虫。
在比赛期间，36%的狗出现腹泻，57.5%的狗出现血便、粪便中有黏液或柏油样便，或同时出现这 3 种症状。比赛前，78.2%的狗和比赛期间 71.3%的狗分离出了沙门氏菌。比赛前，100%的狗和 58.2%的狗分离出了产气荚膜梭菌和艰难梭菌，比赛期间，95%的狗和 36.3%的狗分离出了产气荚膜梭菌和艰难梭菌。与比赛前相比，比赛期间狗 CPE 检测呈阳性的可能性更高（18.8%比 5.5%，P =.021）；然而，没有任何一种肠病原体或其相应的毒素与血便或腹泻有显著相关性。
参加长距离比赛的雪橇犬腹泻和血便的患病率很高，但与常见的肠道病原体无关。可能是长时间的运动对胃肠道造成了影响，导致了腹泻和血便。
