对患有腹泻的犬只进行常规诊断性粪便检查的评估。

Evaluation of a routine diagnostic fecal panel for dogs with diarrhea.

作者信息

Cave Nicholas J, Marks Stanley L, Kass Philip H, Melli Ann C, Brophy Melissa A

机构信息

Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis 95616, USA.

出版信息

J Am Vet Med Assoc. 2002 Jul 1;221(1):52-9. doi: 10.2460/javma.2002.221.52.

DOI:10.2460/javma.2002.221.52

PMID:12420824

Abstract

OBJECTIVES

To assess the diagnostic yield of a routine fecal panel and determine whether Clostridium perfringens or C difficile toxin production is associated with acute hemorrhagic diarrheal syndrome (AHDS) in dogs.

DESIGN

Case-control study.

ANIMALS

260 dogs with diarrhea and 177 dogs with normal feces.

PROCEDURE

Medical records were reviewed for results of culture for C difficile, Campylobacterspp, and Salmonella spp; C perfringens fecal enterotoxin (CPE) assay via ELISA or reverse passive latex agglutination (RPLA) assay; fecal endospore enumeration; C difficile toxin A assay; and parasite evaluation.

RESULTS

Prevalence of CPE in dogs with diarrhea was 22/154 (14.3%) via ELISA and 47/104 (45.2%) via RPLA assay, versus 9/74 (12%) via ELISA and 26/103 (25%) via RPLA assay in control dogs. Prevalence of C difficile was 47/260 (18%) in dogs with diarrhea and 41/74 (55%) in control dogs. Prevalence of C difficile toxin A was 26/254 (10.2%) in dogs with diarrhea and 0/74 in control dogs. Diagnosis of AHDS was made in 27 dogs; 8 had positive results for CPE, 7 had positive results for toxin A, and 1 had positive results for both toxins. Campylobacter spp were isolated from 13 of 260 (5%) dogs with diarrhea and 21 of 74 (28.4%) control dogs. Salmonella spp were isolated from 3 (1.2%) dogs with diarrhea.

CONCLUSIONS AND CLINICAL RELEVANCE

Diagnostic value of a fecal panel in dogs with diarrhea appears to be low.

摘要

目的

评估常规粪便检测的诊断价值，并确定产气荚膜梭菌或艰难梭菌毒素产生是否与犬急性出血性腹泻综合征（AHDS）相关。

设计

病例对照研究。

动物

260只腹泻犬和177只粪便正常的犬。

方法

查阅病历，了解艰难梭菌、弯曲杆菌属和沙门氏菌属的培养结果；通过酶联免疫吸附测定（ELISA）或反向被动乳胶凝集试验（RPLA）检测产气荚膜梭菌粪便肠毒素（CPE）；进行粪便芽孢计数；检测艰难梭菌毒素A；并进行寄生虫评估。

结果

腹泻犬中，通过ELISA检测CPE的患病率为22/154（14.3%），通过RPLA检测为47/104（45.2%）；对照犬中，通过ELISA检测为9/74（12%），通过RPLA检测为26/103（25%）。腹泻犬中艰难梭菌的患病率为47/260（18%），对照犬中为41/74（55%）。腹泻犬中艰难梭菌毒素A的患病率为26/254（10.2%），对照犬中为0/74。27只犬被诊断为AHDS；8只CPE检测呈阳性，7只毒素A检测呈阳性，1只两种毒素检测均呈阳性。260只腹泻犬中有13只（5%）分离出弯曲杆菌属，74只对照犬中有21只（28.4%）分离出弯曲杆菌属。3只（1.2%）腹泻犬分离出沙门氏菌属。