Institute of Entomology, Biology Centre, Academy of Sciences, Branisovská 31, 370 05 Ceské Budejovice, Czech Republic.
Gen Comp Endocrinol. 2010 Apr 1;166(2):330-6. doi: 10.1016/j.ygcen.2009.11.010. Epub 2009 Nov 17.
The RP HPLC and LC/MS QTOF analyses of the methanolic CNS extract from isopod crustacean the woodlouse, Porcellio scaber revealed a presence of the red pigment-concentrating hormone (Panbo-RPCH) in this species. It has been shown that this neuropeptide plays a role in mobilization of energy stores: topical treatments of P. scaber individuals by Panbo-RPCH in a concentration 20 pmol/microl increased the level of glucose in haemolymph about 4 times, while the level of trehalose was only doubled. The results demonstrated that glucose was the main carbohydrate mobilized by the Panbo-RPCH treatment: glucose was responsible for about 97% of total carbohydrate increasing. Despite the demonstration of hyperglycaemic activity of Panbo-RPCH, no stimulatory effect of this hormone on the locomotory activity of P. scaber was observed. The present study is the first discovery of an occurrence of Panbo-RPCH and its hyperglycaemic activity in the representative of the isopod crustaceans. The relationship of the function of Panbo-RPCH in P. scaber to the role of this neuropeptide and adipokinetic hormones in insects is discussed.
从等足目甲壳动物木虱 Porcellio scaber 的甲醇中枢神经系统提取物的 RP HPLC 和 LC/MS QTOF 分析中发现，该物种中存在红色色素浓缩激素 (Panbo-RPCH)。已经表明，这种神经肽在动员能量储存方面发挥作用：用 Panbo-RPCH 以 20 pmol/微升的浓度对 P. scaber 个体进行局部处理，可使血淋巴中的葡萄糖水平增加约 4 倍，而海藻糖水平仅增加了一倍。结果表明，葡萄糖是 Panbo-RPCH 处理动员的主要碳水化合物：葡萄糖约占总碳水化合物增加的 97%。尽管已经证明 Panbo-RPCH 具有升高血糖的活性，但未观察到这种激素对 P. scaber 运动活性的刺激作用。本研究首次发现 Panbo-RPCH 及其在等足目甲壳动物代表中的升血糖活性。讨论了 Panbo-RPCH 在 P. scaber 中的功能与这种神经肽和昆虫中的脂肪激素的作用之间的关系。
