School of Polymer, Textile and Fiber Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Acta Biomater. 2010 Jun;6(6):2003-12. doi: 10.1016/j.actbio.2009.11.007. Epub 2009 Nov 16.
This paper presents the preparation and absorption performance of iodixanol-imprinted polymers in aqueous and blood plasma media in vitro for biomedical applications. The imprinted polymers were prepared by non-covalent imprinting of iodixanol in a matrix of poly(4-vinylpyridine) crosslinked by ethylene glycol dimethacrylate. The binding capacities (BCs) were investigated as a function of template-to-monomer, as well as monomer-to-crosslinker, ratios in the polymerization, and the solvent type. The highest BC of iodixanols achieved from the optimized imprinted polymer in the aqueous solution is 284mgg(-1) dry polymer with an imprinting effect (IE) 8.8 times higher than that of the non-imprinted polymer. In blood plasma, the BC of this polymer is slightly reduced to 232mgg(-1) with a smaller IE 4.3 times higher than that of the control polymer. The BCs of molecularly imprinted polymers as a function of the initial assay solution concentration as well as the examination time are also addressed. Surface analyses were additionally performed to characterize the surface morphologies and porosities of synthetic polymers. This work has demonstrated the feasibility of molecular imprinting of iodixanol, and the observed absorption performance of the imprinted polymers is encouraging for biomedical applications.
本文介绍了碘海醇印迹聚合物在水相和血浆介质中的制备及其在生物医学中的体外吸收性能。印迹聚合物是通过非共价印迹法在聚（4-乙烯基吡啶）交联的乙二醇二甲基丙烯酸酯基质中制备的。研究了模板与单体、单体与交联剂的比例以及溶剂类型对结合容量（BC）的影响。在水溶液中，优化后的印迹聚合物的碘海醇最大 BC 为 284mgg(-1)干聚合物，印迹效应（IE）比非印迹聚合物高 8.8 倍。在血浆中，该聚合物的 BC 略有降低至 232mgg(-1)，IE 比对照聚合物高 4.3 倍。还研究了印迹聚合物的 BC 作为初始测定溶液浓度和检查时间的函数。此外，还进行了表面分析以表征合成聚合物的表面形貌和孔隙率。这项工作证明了碘海醇分子印迹的可行性，并且观察到的印迹聚合物的吸收性能对于生物医学应用是令人鼓舞的。
